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- W2057153221 abstract "Fluorescence in situ hybridization (FISH) has become an indispensable tool for rapid and direct single-cell identification of microbes by detecting signature regions in their rRNA molecules. Recent advances in this field include new web-based tools for assisting probe design and optimization of experimental conditions, easy-to-implement signal amplification strategies, innovative multiplexing approaches, and the combination of FISH with transmission electron microscopy or extracellular staining techniques. Further emerging developments focus on sorting FISH-identified cells for subsequent single-cell genomics and on the direct detection of specific genes within single microbial cells by advanced FISH techniques employing various strategies for massive signal amplification." @default.
- W2057153221 created "2016-06-24" @default.
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- W2057153221 date "2012-02-01" @default.
- W2057153221 modified "2023-10-13" @default.
- W2057153221 title "New trends in fluorescence in situ hybridization for identification and functional analyses of microbes" @default.
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- W2057153221 doi "https://doi.org/10.1016/j.copbio.2011.10.010" @default.
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