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- W2057153942 abstract "The study describes the changes made to adapt the lymphocyte culture technique and the analysis of sister chromatid exchange (SCE ) for South American camelids in the Laboratory of Human Genetics, San Marcos University, Lima, Peru used in the University of Naples and the National Research Centre (CNR- ISPAAM), Italy. Blood samples were collected from 8 alpacas and 19 llamas from Puno and Huancavelica, both sexes, and approximately 5 years old. The results allowed visualizing lymphocyte cells dividing and a mitotic index average of five metaphases per field observed at 100X. The adapted technique is considered of simple application, allowing to estimate the frequency of SCE securely and unambiguously, and it is supported by differential staining of sister chromatids with Giemsa or fluorochrome when incorporated 5-BrdU for two consecutive cell cycles." @default.
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- W2057153942 date "2014-12-30" @default.
- W2057153942 modified "2023-09-27" @default.
- W2057153942 title "Adaptación de la Técnica de Cultivo de Linfocitos de Alpacas y Llamas para el Análisis de Intercambio de Cromátides Hermanas" @default.
- W2057153942 doi "https://doi.org/10.15381/rivep.v25i4.10781" @default.
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