Matches in SemOpenAlex for { <https://semopenalex.org/work/W2057173859> ?p ?o ?g. }
Showing items 1 to 62 of
62
with 100 items per page.
- W2057173859 endingPage "463a" @default.
- W2057173859 startingPage "463a" @default.
- W2057173859 abstract "Central to the human microRNA (miRNA) pathway of gene regulation is the RNA-induced silencing complex (RISC), which is composed of an Argonaute protein bound to a guide strand of single stranded RNA that grants specificity via complementarity to the gene target. The assembly of this ribonucleoprotein depends on the RISC-loading complex (RLC), which comprises the endoribonuclease Dicer, a dsRNA binding protein such as TRBP, and an Argonaute protein. We currently lack mechanistic details regarding the role of TRBP and its homologs in the process of RISC loading. TRBP is known to increase the stability of Dicer and promote interactions between the three protein components of the RLC. An additional hypothesized role for TRBP is to act as a chaperone that shuttles an RNA duplex from Dicer to Argonaute, potentially taking part in the process of strand selection that determines which arm of the double stranded RNA (dsRNA) will be loaded into the RISC to become the guide strand and thus which genes will be targeted. TRBP contains two canonical dsRNA binding domains (dsRBDs) known to bind dsRNA tightly, and a third non-canonical dsRBD that binds instead to Dicer. We used NMR spectroscopy to demonstrate that these three domains do not interact with each other. Using X-ray crystallography, we have determined the structure of the Dicer-TRBP interface, revealing where and how TRBP is anchored in the context of the RLC. Subsequent cellular experiments disrupted this Dicer-TRBP interaction to examine the effects of an absent TRBP on the miRNA processing pathway. Together these results shed light on TRBP's role in the poorly understood processes of RISC-loading and strand selection." @default.
- W2057173859 created "2016-06-24" @default.
- W2057173859 creator A5063620085 @default.
- W2057173859 creator A5067184382 @default.
- W2057173859 date "2014-01-01" @default.
- W2057173859 modified "2023-09-23" @default.
- W2057173859 title "The Dicer-TRBP Interface Structure and Implications for Strand Selection During Microrna Biogenesis" @default.
- W2057173859 doi "https://doi.org/10.1016/j.bpj.2013.11.2623" @default.
- W2057173859 hasPublicationYear "2014" @default.
- W2057173859 type Work @default.
- W2057173859 sameAs 2057173859 @default.
- W2057173859 citedByCount "1" @default.
- W2057173859 countsByYear W20571738592020 @default.
- W2057173859 crossrefType "journal-article" @default.
- W2057173859 hasAuthorship W2057173859A5063620085 @default.
- W2057173859 hasAuthorship W2057173859A5067184382 @default.
- W2057173859 hasBestOaLocation W20571738591 @default.
- W2057173859 hasConcept C104317684 @default.
- W2057173859 hasConcept C155330019 @default.
- W2057173859 hasConcept C166703698 @default.
- W2057173859 hasConcept C201853390 @default.
- W2057173859 hasConcept C2191507 @default.
- W2057173859 hasConcept C2776802755 @default.
- W2057173859 hasConcept C2779867133 @default.
- W2057173859 hasConcept C41282012 @default.
- W2057173859 hasConcept C54355233 @default.
- W2057173859 hasConcept C67705224 @default.
- W2057173859 hasConcept C86803240 @default.
- W2057173859 hasConcept C95444343 @default.
- W2057173859 hasConceptScore W2057173859C104317684 @default.
- W2057173859 hasConceptScore W2057173859C155330019 @default.
- W2057173859 hasConceptScore W2057173859C166703698 @default.
- W2057173859 hasConceptScore W2057173859C201853390 @default.
- W2057173859 hasConceptScore W2057173859C2191507 @default.
- W2057173859 hasConceptScore W2057173859C2776802755 @default.
- W2057173859 hasConceptScore W2057173859C2779867133 @default.
- W2057173859 hasConceptScore W2057173859C41282012 @default.
- W2057173859 hasConceptScore W2057173859C54355233 @default.
- W2057173859 hasConceptScore W2057173859C67705224 @default.
- W2057173859 hasConceptScore W2057173859C86803240 @default.
- W2057173859 hasConceptScore W2057173859C95444343 @default.
- W2057173859 hasIssue "2" @default.
- W2057173859 hasLocation W20571738591 @default.
- W2057173859 hasOpenAccess W2057173859 @default.
- W2057173859 hasPrimaryLocation W20571738591 @default.
- W2057173859 hasRelatedWork W1500039090 @default.
- W2057173859 hasRelatedWork W1953646178 @default.
- W2057173859 hasRelatedWork W1990135880 @default.
- W2057173859 hasRelatedWork W2028377604 @default.
- W2057173859 hasRelatedWork W2111436354 @default.
- W2057173859 hasRelatedWork W2160388811 @default.
- W2057173859 hasRelatedWork W2383848441 @default.
- W2057173859 hasRelatedWork W2495626116 @default.
- W2057173859 hasRelatedWork W2778093784 @default.
- W2057173859 hasRelatedWork W76225061 @default.
- W2057173859 hasVolume "106" @default.
- W2057173859 isParatext "false" @default.
- W2057173859 isRetracted "false" @default.
- W2057173859 magId "2057173859" @default.
- W2057173859 workType "article" @default.