Matches in SemOpenAlex for { <https://semopenalex.org/work/W2057183055> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2057183055 endingPage "447a" @default.
- W2057183055 startingPage "447a" @default.
- W2057183055 abstract "At the interface of extracellular-substrate interactions, substrate elasticities strongly influence cell function and morphology. This finding is important in diverse areas including neural function, metastasis, and heart diseases. To address the effects of localized elasticity, we developed a new method to control the microenvironment through generating a substrate with localized alternating stiffnesses interacting with cells. We first fabricated polymeric microchannels (30μm and 100μm wide, both 50μm deep) through using conventional soft lithography with poly(dimethylsiloxane) (PDMS) and a 5:1-base/curing agent. We then poured 30:1-base/curing PDMS into the channels to produce alternating surfaces with elasticities of 800kPa and 200kPa adjacent to one another. We used these substrates and independently seeded NIH 3T3 fibroblasts and Neuro2A neuroblasts onto the systems to analyze their morphologies and structure. We found that fibroblasts attached on the soft surface had smaller projected area, lower cell density, and multiple cells versus single cell differences. Also, the neuroblasts had distinct patterns of neural outgrowth that emerged as they extended processes to these alternating stiffness substrates, depending on the location of the soma and the surface stiffness. We believe further use of this approach will promote greater understanding of cellular responses as well as insights into a variety of diseases that can be investigated using controlled cell-material interactions and substrate elasticities." @default.
- W2057183055 created "2016-06-24" @default.
- W2057183055 creator A5017695421 @default.
- W2057183055 creator A5019672982 @default.
- W2057183055 creator A5074912291 @default.
- W2057183055 creator A5075207633 @default.
- W2057183055 date "2011-02-01" @default.
- W2057183055 modified "2023-09-29" @default.
- W2057183055 title "Cell Structure and Morphology Alterations Through Controlling Localized Substrate Stiffness on in Fibroblasts and Neuroblasts" @default.
- W2057183055 doi "https://doi.org/10.1016/j.bpj.2010.12.2634" @default.
- W2057183055 hasPublicationYear "2011" @default.
- W2057183055 type Work @default.
- W2057183055 sameAs 2057183055 @default.
- W2057183055 citedByCount "0" @default.
- W2057183055 crossrefType "journal-article" @default.
- W2057183055 hasAuthorship W2057183055A5017695421 @default.
- W2057183055 hasAuthorship W2057183055A5019672982 @default.
- W2057183055 hasAuthorship W2057183055A5074912291 @default.
- W2057183055 hasAuthorship W2057183055A5075207633 @default.
- W2057183055 hasBestOaLocation W20571830551 @default.
- W2057183055 hasConcept C121854251 @default.
- W2057183055 hasConcept C12554922 @default.
- W2057183055 hasConcept C1491633281 @default.
- W2057183055 hasConcept C159985019 @default.
- W2057183055 hasConcept C171250308 @default.
- W2057183055 hasConcept C175369904 @default.
- W2057183055 hasConcept C185592680 @default.
- W2057183055 hasConcept C18903297 @default.
- W2057183055 hasConcept C192562407 @default.
- W2057183055 hasConcept C2777289219 @default.
- W2057183055 hasConcept C2779372316 @default.
- W2057183055 hasConcept C39961270 @default.
- W2057183055 hasConcept C4746552 @default.
- W2057183055 hasConcept C54355233 @default.
- W2057183055 hasConcept C55493867 @default.
- W2057183055 hasConcept C81885089 @default.
- W2057183055 hasConcept C86803240 @default.
- W2057183055 hasConcept C95444343 @default.
- W2057183055 hasConceptScore W2057183055C121854251 @default.
- W2057183055 hasConceptScore W2057183055C12554922 @default.
- W2057183055 hasConceptScore W2057183055C1491633281 @default.
- W2057183055 hasConceptScore W2057183055C159985019 @default.
- W2057183055 hasConceptScore W2057183055C171250308 @default.
- W2057183055 hasConceptScore W2057183055C175369904 @default.
- W2057183055 hasConceptScore W2057183055C185592680 @default.
- W2057183055 hasConceptScore W2057183055C18903297 @default.
- W2057183055 hasConceptScore W2057183055C192562407 @default.
- W2057183055 hasConceptScore W2057183055C2777289219 @default.
- W2057183055 hasConceptScore W2057183055C2779372316 @default.
- W2057183055 hasConceptScore W2057183055C39961270 @default.
- W2057183055 hasConceptScore W2057183055C4746552 @default.
- W2057183055 hasConceptScore W2057183055C54355233 @default.
- W2057183055 hasConceptScore W2057183055C55493867 @default.
- W2057183055 hasConceptScore W2057183055C81885089 @default.
- W2057183055 hasConceptScore W2057183055C86803240 @default.
- W2057183055 hasConceptScore W2057183055C95444343 @default.
- W2057183055 hasIssue "3" @default.
- W2057183055 hasLocation W20571830551 @default.
- W2057183055 hasOpenAccess W2057183055 @default.
- W2057183055 hasPrimaryLocation W20571830551 @default.
- W2057183055 hasRelatedWork W1964523160 @default.
- W2057183055 hasRelatedWork W1983744704 @default.
- W2057183055 hasRelatedWork W1983746021 @default.
- W2057183055 hasRelatedWork W1988124945 @default.
- W2057183055 hasRelatedWork W1992976697 @default.
- W2057183055 hasRelatedWork W2030231709 @default.
- W2057183055 hasRelatedWork W2049878449 @default.
- W2057183055 hasRelatedWork W2056330129 @default.
- W2057183055 hasRelatedWork W2067775806 @default.
- W2057183055 hasRelatedWork W2107711785 @default.
- W2057183055 hasRelatedWork W2264755416 @default.
- W2057183055 hasRelatedWork W2304643177 @default.
- W2057183055 hasRelatedWork W2307147706 @default.
- W2057183055 hasRelatedWork W2549198633 @default.
- W2057183055 hasRelatedWork W2796072630 @default.
- W2057183055 hasRelatedWork W2796581813 @default.
- W2057183055 hasRelatedWork W2946134573 @default.
- W2057183055 hasRelatedWork W2953174209 @default.
- W2057183055 hasRelatedWork W2965352456 @default.
- W2057183055 hasRelatedWork W2987259165 @default.
- W2057183055 hasVolume "100" @default.
- W2057183055 isParatext "false" @default.
- W2057183055 isRetracted "false" @default.
- W2057183055 magId "2057183055" @default.
- W2057183055 workType "article" @default.