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• W2057188601 abstract "Spodoptera frugiperda β-1,3-glucanase (SLam) was purified from larval midgut. It has a molecular mass of 37.5 kDa, an alkaline optimum pH of 9.0, is active against β-1,3-glucan (laminarin), but cannot hydrolyze yeast β-1,3-1,6-glucan or other polysaccharides. The enzyme is an endoglucanase with low processivity (0.4), and is not inhibited by high concentrations of substrate. In contrast to other digestive β-1,3-glucanases from insects, SLam is unable to lyse Saccharomyces cerevisae cells. The cDNA encoding SLam was cloned and sequenced, showing that the protein belongs to glycosyl hydrolase family 16 as other insect glucanases and glucan-binding proteins. Multiple sequence alignment of β-1,3-glucanases and β-glucan-binding protein supports the assumption that the β-1,3-glucanase gene duplicated in the ancestor of mollusks and arthropods. One copy originated the derived β-1,3-glucanases by the loss of an extended N-terminal region and the β-glucan-binding proteins by the loss of the catalytic residues. SLam homology modeling suggests that E228 may affect the ionization of the catalytic residues, thus displacing the enzyme pH optimum. SLam antiserum reacts with a single protein in the insect midgut. Immunocytolocalization shows that the enzyme is present in secretory vesicles and glycocalyx from columnar cells." @default.
• W2057188601 created "2016-06-24" @default.
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• W2057188601 date "2010-12-01" @default.
• W2057188601 modified "2023-09-30" @default.
• W2057188601 title "Characterization of a β-1,3-glucanase active in the alkaline midgut of Spodoptera frugiperda larvae and its relation to β-glucan-binding proteins" @default.
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• W2057188601 cites W1986191025 @default.
• W2057188601 cites W1987250042 @default.
• W2057188601 cites W1990031215 @default.
• W2057188601 cites W1992907342 @default.
• W2057188601 cites W1996663173 @default.
• W2057188601 cites W2012975084 @default.
• W2057188601 cites W2013713775 @default.
• W2057188601 cites W2013925839 @default.
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• W2057188601 doi "https://doi.org/10.1016/j.ibmb.2010.08.006" @default.
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