Matches in SemOpenAlex for { <https://semopenalex.org/work/W2057203476> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2057203476 endingPage "484" @default.
- W2057203476 startingPage "480" @default.
- W2057203476 abstract "Both estrogen receptor-positive (ER+), tamoxifen-sensitive (5–21) and tamoxifen-resistant (5–23) subclones of the parental MCF-7 breast cancer cell line were used in competitive ligand binding studies involving either [3H]ICI 182,780 or 4-OH-[3H]tamoxifen (4OHT) displacement by unlabelled estradiol (E2) or the antiestrogens (AE) 4OHT and ICI 182,780. Neither radioligand was displaced significantly by E2 over a range of concentrations; binding was predominantly inhibited by the corresponding radio-inert ligand. Scatchard analysis of the data revealed that the binding capacities of both cell lines for ICI 182,780 were approximately 7-fold greater than the previously determined number of ER sites per cell, with the affinity being an order of magnitude less than that of E2 for ER. No difference was found between the TAM-sensitive and -resistant cells in their binding of either AE. When cells were preincubated with either E2, TAM or 4OHT at a high, fixed concentration to block the ER or AE binding sites (AEBS), respectively, displaceable binding of [3H]ICI 182,780 was still observed, indicating binding at a site other than the classical ER or previously described AEBS. Our results suggest that there is a specific, saturable and relatively high-affinity binding site for ICI 182,780 in MCF 5–21 and MCF 5–23 breast cancer cells. However, the physiological relevance of this binding site requires further clarification because in cell growth assays, E2 (at the dose of ICI 182,780) overcame the inhibitory effect of the antiestrogen in both of the cell lines. © 1995 Wiley-Liss, Inc." @default.
- W2057203476 created "2016-06-24" @default.
- W2057203476 creator A5004548801 @default.
- W2057203476 creator A5008173055 @default.
- W2057203476 creator A5027248689 @default.
- W2057203476 creator A5027365975 @default.
- W2057203476 creator A5055335209 @default.
- W2057203476 creator A5076032059 @default.
- W2057203476 date "1995-08-09" @default.
- W2057203476 modified "2023-09-27" @default.
- W2057203476 title "The pure antiestrogen ICI 182,780 binds to a high-affinity site distinct from the estrogen receptor" @default.
- W2057203476 cites W1595603156 @default.
- W2057203476 cites W1646234539 @default.
- W2057203476 cites W1975402264 @default.
- W2057203476 cites W1981959249 @default.
- W2057203476 cites W1983649348 @default.
- W2057203476 cites W2019201668 @default.
- W2057203476 cites W2038657843 @default.
- W2057203476 cites W2043813981 @default.
- W2057203476 cites W2044469448 @default.
- W2057203476 cites W206480088 @default.
- W2057203476 cites W2074335085 @default.
- W2057203476 cites W2153285273 @default.
- W2057203476 cites W2338351455 @default.
- W2057203476 doi "https://doi.org/10.1002/ijc.2910620420" @default.
- W2057203476 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7635575" @default.
- W2057203476 hasPublicationYear "1995" @default.
- W2057203476 type Work @default.
- W2057203476 sameAs 2057203476 @default.
- W2057203476 citedByCount "32" @default.
- W2057203476 countsByYear W20572034762013 @default.
- W2057203476 countsByYear W20572034762014 @default.
- W2057203476 countsByYear W20572034762018 @default.
- W2057203476 crossrefType "journal-article" @default.
- W2057203476 hasAuthorship W2057203476A5004548801 @default.
- W2057203476 hasAuthorship W2057203476A5008173055 @default.
- W2057203476 hasAuthorship W2057203476A5027248689 @default.
- W2057203476 hasAuthorship W2057203476A5027365975 @default.
- W2057203476 hasAuthorship W2057203476A5055335209 @default.
- W2057203476 hasAuthorship W2057203476A5076032059 @default.
- W2057203476 hasConcept C107824862 @default.
- W2057203476 hasConcept C116569031 @default.
- W2057203476 hasConcept C121608353 @default.
- W2057203476 hasConcept C126322002 @default.
- W2057203476 hasConcept C134018914 @default.
- W2057203476 hasConcept C170493617 @default.
- W2057203476 hasConcept C185592680 @default.
- W2057203476 hasConcept C2776067312 @default.
- W2057203476 hasConcept C2777164284 @default.
- W2057203476 hasConcept C2777176818 @default.
- W2057203476 hasConcept C2909005528 @default.
- W2057203476 hasConcept C530470458 @default.
- W2057203476 hasConcept C55493867 @default.
- W2057203476 hasConcept C67847695 @default.
- W2057203476 hasConcept C71924100 @default.
- W2057203476 hasConcept C84606932 @default.
- W2057203476 hasConcept C86803240 @default.
- W2057203476 hasConceptScore W2057203476C107824862 @default.
- W2057203476 hasConceptScore W2057203476C116569031 @default.
- W2057203476 hasConceptScore W2057203476C121608353 @default.
- W2057203476 hasConceptScore W2057203476C126322002 @default.
- W2057203476 hasConceptScore W2057203476C134018914 @default.
- W2057203476 hasConceptScore W2057203476C170493617 @default.
- W2057203476 hasConceptScore W2057203476C185592680 @default.
- W2057203476 hasConceptScore W2057203476C2776067312 @default.
- W2057203476 hasConceptScore W2057203476C2777164284 @default.
- W2057203476 hasConceptScore W2057203476C2777176818 @default.
- W2057203476 hasConceptScore W2057203476C2909005528 @default.
- W2057203476 hasConceptScore W2057203476C530470458 @default.
- W2057203476 hasConceptScore W2057203476C55493867 @default.
- W2057203476 hasConceptScore W2057203476C67847695 @default.
- W2057203476 hasConceptScore W2057203476C71924100 @default.
- W2057203476 hasConceptScore W2057203476C84606932 @default.
- W2057203476 hasConceptScore W2057203476C86803240 @default.
- W2057203476 hasIssue "4" @default.
- W2057203476 hasLocation W20572034761 @default.
- W2057203476 hasLocation W20572034762 @default.
- W2057203476 hasOpenAccess W2057203476 @default.
- W2057203476 hasPrimaryLocation W20572034761 @default.
- W2057203476 hasRelatedWork W2015860573 @default.
- W2057203476 hasRelatedWork W2047760789 @default.
- W2057203476 hasRelatedWork W2057203476 @default.
- W2057203476 hasRelatedWork W2068408721 @default.
- W2057203476 hasRelatedWork W2093476509 @default.
- W2057203476 hasRelatedWork W2101821182 @default.
- W2057203476 hasRelatedWork W2150216118 @default.
- W2057203476 hasRelatedWork W2161550802 @default.
- W2057203476 hasRelatedWork W2926335606 @default.
- W2057203476 hasRelatedWork W3141193604 @default.
- W2057203476 hasVolume "62" @default.
- W2057203476 isParatext "false" @default.
- W2057203476 isRetracted "false" @default.
- W2057203476 magId "2057203476" @default.
- W2057203476 workType "article" @default.