FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2057257531> ?p ?o ?g. }

• W2057257531 endingPage "e105167" @default.
• W2057257531 startingPage "e105167" @default.
• W2057257531 abstract "Rotavirus virus-like particles (RV-VLPs) are potential alternative non-live vaccine candidates due to their high immunogenicity. They mimic the natural conformation of native viral proteins but cannot replicate because they do not contain genomic material which makes them safe. To date, most RV-VLPs have been derived from cell culture adapted strains or common G1 and G3 rotaviruses that have been circulating in communities for some time. In this study, chimaeric RV-VLPs were generated from the consensus sequences of African rotaviruses (G2, G8, G9 or G12 strains associated with either P[4], P[6] or P[8] genotypes) characterised directly from human stool samples without prior adaptation of the wild type strains to cell culture. Codon-optimised sequences for insect cell expression of genome segments 2 (VP2), 4 (VP4), 6 (VP6) and 9 (VP7) were cloned into a modified pFASTBAC vector, which allowed simultaneous expression of up to four genes using the Bac-to-Bac Baculovirus Expression System (BEVS; Invitrogen). Several combinations of the genome segments originating from different field strains were cloned to produce double-layered RV-VLPs (dRV-VLP; VP2/6), triple-layered RV-VLPs (tRV-VLP; VP2/6/7 or VP2/6/7/4) and chimaeric tRV-VLPs. The RV-VLPs were produced by infecting Spodoptera frugiperda 9 and Trichoplusia ni cells with recombinant baculoviruses using multi-cistronic, dual co-infection and stepwise-infection expression strategies. The size and morphology of the RV-VLPs, as determined by transmission electron microscopy, revealed successful production of RV-VLPs. The novel approach of producing tRV-VLPs, by using the consensus insect cell codon-optimised nucleotide sequence derived from dsRNA extracted directly from clinical specimens, should speed-up vaccine research and development by by-passing the need to adapt rotaviruses to cell culture. Other problems associated with cell culture adaptation, such as possible changes in epitopes, can also be circumvented. Thus, it is now possible to generate tRV-VLPs for evaluation as non-live vaccine candidates for any human or animal field rotavirus strain." @default.
• W2057257531 created "2016-06-24" @default.
• W2057257531 creator A5008002688 @default.
• W2057257531 creator A5049747325 @default.
• W2057257531 creator A5076387327 @default.
• W2057257531 creator A5081589040 @default.
• W2057257531 date "2014-09-30" @default.
• W2057257531 modified "2023-09-27" @default.
• W2057257531 title "Chimaeric Virus-Like Particles Derived from Consensus Genome Sequences of Human Rotavirus Strains Co-Circulating in Africa" @default.
• W2057257531 cites W148109975 @default.
• W2057257531 cites W1483515383 @default.
• W2057257531 cites W1532585732 @default.
• W2057257531 cites W1568396084 @default.
• W2057257531 cites W1585061035 @default.
• W2057257531 cites W1676066429 @default.
• W2057257531 cites W1838465562 @default.
• W2057257531 cites W1901371944 @default.
• W2057257531 cites W1927716204 @default.
• W2057257531 cites W1938711200 @default.
• W2057257531 cites W1964196308 @default.
• W2057257531 cites W1965553991 @default.
• W2057257531 cites W1974895422 @default.
• W2057257531 cites W1975640964 @default.
• W2057257531 cites W1975818001 @default.
• W2057257531 cites W1976630772 @default.
• W2057257531 cites W1985178009 @default.
• W2057257531 cites W1988275534 @default.
• W2057257531 cites W2011175996 @default.
• W2057257531 cites W2020174558 @default.
• W2057257531 cites W2027433479 @default.
• W2057257531 cites W2029794641 @default.
• W2057257531 cites W2029854306 @default.
• W2057257531 cites W2029926070 @default.
• W2057257531 cites W2037854094 @default.
• W2057257531 cites W2039739473 @default.
• W2057257531 cites W2047567108 @default.
• W2057257531 cites W2049505598 @default.
• W2057257531 cites W2052873381 @default.
• W2057257531 cites W2053413274 @default.
• W2057257531 cites W2053752274 @default.
• W2057257531 cites W2054068279 @default.
• W2057257531 cites W2056321947 @default.
• W2057257531 cites W2066799171 @default.
• W2057257531 cites W2075515367 @default.
• W2057257531 cites W2080833698 @default.
• W2057257531 cites W2083240451 @default.
• W2057257531 cites W2085012067 @default.
• W2057257531 cites W2087144533 @default.
• W2057257531 cites W2088634892 @default.
• W2057257531 cites W2089190345 @default.
• W2057257531 cites W2092725573 @default.
• W2057257531 cites W2095820230 @default.
• W2057257531 cites W2097696280 @default.
• W2057257531 cites W2100837269 @default.
• W2057257531 cites W2101134528 @default.
• W2057257531 cites W2104964894 @default.
• W2057257531 cites W2107646436 @default.
• W2057257531 cites W2108945812 @default.
• W2057257531 cites W2113014022 @default.
• W2057257531 cites W2123656421 @default.
• W2057257531 cites W2131154800 @default.
• W2057257531 cites W2133762643 @default.
• W2057257531 cites W2135437427 @default.
• W2057257531 cites W2135985984 @default.
• W2057257531 cites W2143084803 @default.
• W2057257531 cites W2143233237 @default.
• W2057257531 cites W2144868386 @default.
• W2057257531 cites W2146019635 @default.
• W2057257531 cites W2149462395 @default.
• W2057257531 cites W2156167673 @default.
• W2057257531 cites W2162190408 @default.
• W2057257531 cites W2165128843 @default.
• W2057257531 cites W2165691918 @default.
• W2057257531 cites W2166645516 @default.
• W2057257531 cites W2170530187 @default.
• W2057257531 cites W2170749337 @default.
• W2057257531 cites W4231603137 @default.
• W2057257531 doi "https://doi.org/10.1371/journal.pone.0105167" @default.
• W2057257531 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4181975" @default.
• W2057257531 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25268783" @default.
• W2057257531 hasPublicationYear "2014" @default.
• W2057257531 type Work @default.
• W2057257531 sameAs 2057257531 @default.
• W2057257531 citedByCount "5" @default.
• W2057257531 countsByYear W20572575312015 @default.
• W2057257531 countsByYear W20572575312017 @default.
• W2057257531 countsByYear W20572575312018 @default.
• W2057257531 countsByYear W20572575312021 @default.
• W2057257531 countsByYear W20572575312023 @default.
• W2057257531 crossrefType "journal-article" @default.
• W2057257531 hasAuthorship W2057257531A5008002688 @default.
• W2057257531 hasAuthorship W2057257531A5049747325 @default.
• W2057257531 hasAuthorship W2057257531A5076387327 @default.
• W2057257531 hasAuthorship W2057257531A5081589040 @default.
• W2057257531 hasBestOaLocation W20572575311 @default.
• W2057257531 hasConcept C104317684 @default.
• W2057257531 hasConcept C141231307 @default.
• W2057257531 hasConcept C153911025 @default.

• next