FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2057309715> ?p ?o ?g. }

• W2057309715 endingPage "28169" @default.
• W2057309715 startingPage "28160" @default.
• W2057309715 abstract "Importin α1 can bind classical nuclear localization signals (NLSs) in two NLS-binding sites, known as “major” and “minor.” The major site is located between ARM repeats 2–4, whereas the minor site spans ARM 7–8. In this study, we have characterized the cellular localization of human phospholipid scramblase 4 (hPLSCR4), a member of the phospholipid scramblase protein family. We identified a minimal NLS in hPLSCR4 (273GSIIRKWN280) that contains only two basic amino acids. This NLS is both necessary for nuclear localization of hPLSCR4 in transfected HeLa cells and sufficient for nuclear import of a non-diffusible cargo in permeabilized cells. Mutation of only one of the two basic residues, Arg277, correlates with loss of nuclear localization, suggesting this amino acid plays a key role in nuclear transport. Crystallographic analysis of mammalian importin α1 in complex with the hPLSCR4-NLS reveals this minimal NLS binds specifically and exclusively to the minor binding site of importin α. These data provide the first structural and functional evidence of a novel NLS-binding mode in importin α1 that uses only the minor groove as the exclusive site for nuclear import of nonclassical cargos. Importin α1 can bind classical nuclear localization signals (NLSs) in two NLS-binding sites, known as “major” and “minor.” The major site is located between ARM repeats 2–4, whereas the minor site spans ARM 7–8. In this study, we have characterized the cellular localization of human phospholipid scramblase 4 (hPLSCR4), a member of the phospholipid scramblase protein family. We identified a minimal NLS in hPLSCR4 (273GSIIRKWN280) that contains only two basic amino acids. This NLS is both necessary for nuclear localization of hPLSCR4 in transfected HeLa cells and sufficient for nuclear import of a non-diffusible cargo in permeabilized cells. Mutation of only one of the two basic residues, Arg277, correlates with loss of nuclear localization, suggesting this amino acid plays a key role in nuclear transport. Crystallographic analysis of mammalian importin α1 in complex with the hPLSCR4-NLS reveals this minimal NLS binds specifically and exclusively to the minor binding site of importin α. These data provide the first structural and functional evidence of a novel NLS-binding mode in importin α1 that uses only the minor groove as the exclusive site for nuclear import of nonclassical cargos." @default.
• W2057309715 created "2016-06-24" @default.
• W2057309715 creator A5014076963 @default.
• W2057309715 creator A5026350256 @default.
• W2057309715 creator A5029499085 @default.
• W2057309715 creator A5042025565 @default.
• W2057309715 date "2011-08-01" @default.
• W2057309715 modified "2023-10-18" @default.
• W2057309715 title "A Minimal Nuclear Localization Signal (NLS) in Human Phospholipid Scramblase 4 That Binds Only the Minor NLS-binding Site of Importin α1" @default.
• W2057309715 cites W1870505444 @default.
• W2057309715 cites W1967186789 @default.
• W2057309715 cites W1968188734 @default.
• W2057309715 cites W1972217672 @default.
• W2057309715 cites W1973731625 @default.
• W2057309715 cites W1976572901 @default.
• W2057309715 cites W1995160911 @default.
• W2057309715 cites W2001641653 @default.
• W2057309715 cites W2005053655 @default.
• W2057309715 cites W2017119471 @default.
• W2057309715 cites W2032258002 @default.
• W2057309715 cites W2033386104 @default.
• W2057309715 cites W2033722975 @default.
• W2057309715 cites W2035734488 @default.
• W2057309715 cites W2044570539 @default.
• W2057309715 cites W2044673780 @default.
• W2057309715 cites W2046074884 @default.
• W2057309715 cites W2048492672 @default.
• W2057309715 cites W2049132410 @default.
• W2057309715 cites W2054525948 @default.
• W2057309715 cites W2062807911 @default.
• W2057309715 cites W2069512161 @default.
• W2057309715 cites W2071085094 @default.
• W2057309715 cites W2072219433 @default.
• W2057309715 cites W2073526608 @default.
• W2057309715 cites W2075413744 @default.
• W2057309715 cites W2081580769 @default.
• W2057309715 cites W2082389145 @default.
• W2057309715 cites W2085186887 @default.
• W2057309715 cites W2092544515 @default.
• W2057309715 cites W2105957970 @default.
• W2057309715 cites W2108442683 @default.
• W2057309715 cites W2111802043 @default.
• W2057309715 cites W2123986453 @default.
• W2057309715 cites W2127505727 @default.
• W2057309715 cites W2143566830 @default.
• W2057309715 cites W2144081223 @default.
• W2057309715 cites W2147008614 @default.
• W2057309715 cites W2155754742 @default.
• W2057309715 cites W2156891333 @default.
• W2057309715 cites W2156961771 @default.
• W2057309715 cites W2163341755 @default.
• W2057309715 cites W2164410061 @default.
• W2057309715 cites W2180229411 @default.
• W2057309715 cites W2403358826 @default.
• W2057309715 cites W2750896950 @default.
• W2057309715 doi "https://doi.org/10.1074/jbc.m111.228007" @default.
• W2057309715 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3151061" @default.
• W2057309715 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21690087" @default.
• W2057309715 hasPublicationYear "2011" @default.
• W2057309715 type Work @default.
• W2057309715 sameAs 2057309715 @default.
• W2057309715 citedByCount "56" @default.
• W2057309715 countsByYear W20573097152012 @default.
• W2057309715 countsByYear W20573097152013 @default.
• W2057309715 countsByYear W20573097152014 @default.
• W2057309715 countsByYear W20573097152015 @default.
• W2057309715 countsByYear W20573097152016 @default.
• W2057309715 countsByYear W20573097152017 @default.
• W2057309715 countsByYear W20573097152018 @default.
• W2057309715 countsByYear W20573097152019 @default.
• W2057309715 countsByYear W20573097152020 @default.
• W2057309715 countsByYear W20573097152021 @default.
• W2057309715 countsByYear W20573097152022 @default.
• W2057309715 countsByYear W20573097152023 @default.
• W2057309715 crossrefType "journal-article" @default.
• W2057309715 hasAuthorship W2057309715A5014076963 @default.
• W2057309715 hasAuthorship W2057309715A5026350256 @default.
• W2057309715 hasAuthorship W2057309715A5029499085 @default.
• W2057309715 hasAuthorship W2057309715A5042025565 @default.
• W2057309715 hasBestOaLocation W20573097151 @default.
• W2057309715 hasConcept C133882971 @default.
• W2057309715 hasConcept C186435350 @default.
• W2057309715 hasConcept C188810444 @default.
• W2057309715 hasConcept C190062978 @default.
• W2057309715 hasConcept C2778918659 @default.
• W2057309715 hasConcept C2779637612 @default.
• W2057309715 hasConcept C2780114586 @default.
• W2057309715 hasConcept C41625074 @default.
• W2057309715 hasConcept C49805395 @default.
• W2057309715 hasConcept C55493867 @default.
• W2057309715 hasConcept C59157529 @default.
• W2057309715 hasConcept C86803240 @default.
• W2057309715 hasConcept C95444343 @default.
• W2057309715 hasConceptScore W2057309715C133882971 @default.
• W2057309715 hasConceptScore W2057309715C186435350 @default.
• W2057309715 hasConceptScore W2057309715C188810444 @default.
• W2057309715 hasConceptScore W2057309715C190062978 @default.
• W2057309715 hasConceptScore W2057309715C2778918659 @default.

• next