FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2057348102> ?p ?o ?g. }

• W2057348102 endingPage "688" @default.
• W2057348102 startingPage "679" @default.
• W2057348102 abstract "Background In previous studies, we showed that gene activated matrices (GAMs) containing nonviral vectors successfully deliver genes to neurons after optic nerve and spinal cord injury. In the present study, we evaluated whether adenoviral vectors delivered within a GAM increase the efficiency of local gene delivery to injured CNS neurons. Lyophilized GAMs containing collagen and adenoviral vectors were assessed in vitro and in vivo. Methods We evaluated viral vector stability, release kinetics and efficiency of transduction for this GAM formulation in vitro using the quantitative polymerase chain reaction (qPCR), flow cytometry and fluorescence microscopy. Using PCR, reverse transcriptase-PCR and confocal microscopy, we assessed viral DNA retrograde axonal transport, green fluorescent protein (GFP) expression in retinal ganglion cells (RGCs) after GAM implantation into the wound of the rat transected optic nerve. Results qPCR analyses demonstrated that 100% of viral particles were retained within the collagen after lyophilization. In vitro studies demonstrated that 60% of the particles within the GAM were infective and not released from the collagen matrix when placed in water. By 24 h, GFP expression was detected within cells that have invaded the GAM. In vivo studies demonstrated that adenoviral particles were retrogradely transported in axons from the GAM implanted at the lesion site to the RGC in the retina where the corresponding mRNA was expressed. Analysis of the efficiency of cell transduction indicated that 69% of RGC express GFP. Conclusions These studies demonstrate that lyophilized GAMs containing adenoviral particles within collagen are stable, retain a significant proportion of their infectivity and successfully and efficiently deliver genes to neurons after central nervous system injury. Copyright © 2009 John Wiley & Sons, Ltd." @default.
• W2057348102 created "2016-06-24" @default.
• W2057348102 creator A5001537127 @default.
• W2057348102 creator A5004194487 @default.
• W2057348102 creator A5013902407 @default.
• W2057348102 creator A5017942355 @default.
• W2057348102 creator A5024646408 @default.
• W2057348102 creator A5024786945 @default.
• W2057348102 creator A5035294930 @default.
• W2057348102 creator A5059122102 @default.
• W2057348102 creator A5077140361 @default.
• W2057348102 date "2009-08-01" @default.
• W2057348102 modified "2023-10-05" @default.
• W2057348102 title "The deployment of adenovirus-containing gene activated matrices onto severed axons after central nervous system injury leads to transgene expression in target neuronal cell bodies" @default.
• W2057348102 cites W1863551998 @default.
• W2057348102 cites W1979181108 @default.
• W2057348102 cites W1982945073 @default.
• W2057348102 cites W1983025467 @default.
• W2057348102 cites W1983160604 @default.
• W2057348102 cites W1999505861 @default.
• W2057348102 cites W2002454921 @default.
• W2057348102 cites W2003096295 @default.
• W2057348102 cites W2009546242 @default.
• W2057348102 cites W2020340848 @default.
• W2057348102 cites W2027393834 @default.
• W2057348102 cites W2027759472 @default.
• W2057348102 cites W2028997635 @default.
• W2057348102 cites W2032755224 @default.
• W2057348102 cites W2035461375 @default.
• W2057348102 cites W2039422279 @default.
• W2057348102 cites W2048953807 @default.
• W2057348102 cites W2083430062 @default.
• W2057348102 cites W2084891008 @default.
• W2057348102 cites W2094549522 @default.
• W2057348102 cites W2120856340 @default.
• W2057348102 cites W33441858 @default.
• W2057348102 cites W4239159470 @default.
• W2057348102 doi "https://doi.org/10.1002/jgm.1354" @default.
• W2057348102 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4241242" @default.
• W2057348102 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19507166" @default.
• W2057348102 hasPublicationYear "2009" @default.
• W2057348102 type Work @default.
• W2057348102 sameAs 2057348102 @default.
• W2057348102 citedByCount "10" @default.
• W2057348102 countsByYear W20573481022013 @default.
• W2057348102 countsByYear W20573481022014 @default.
• W2057348102 countsByYear W20573481022015 @default.
• W2057348102 countsByYear W20573481022019 @default.
• W2057348102 countsByYear W20573481022023 @default.
• W2057348102 crossrefType "journal-article" @default.
• W2057348102 hasAuthorship W2057348102A5001537127 @default.
• W2057348102 hasAuthorship W2057348102A5004194487 @default.
• W2057348102 hasAuthorship W2057348102A5013902407 @default.
• W2057348102 hasAuthorship W2057348102A5017942355 @default.
• W2057348102 hasAuthorship W2057348102A5024646408 @default.
• W2057348102 hasAuthorship W2057348102A5024786945 @default.
• W2057348102 hasAuthorship W2057348102A5035294930 @default.
• W2057348102 hasAuthorship W2057348102A5059122102 @default.
• W2057348102 hasAuthorship W2057348102A5077140361 @default.
• W2057348102 hasBestOaLocation W20573481022 @default.
• W2057348102 hasConcept C102230213 @default.
• W2057348102 hasConcept C104317684 @default.
• W2057348102 hasConcept C105702510 @default.
• W2057348102 hasConcept C111599444 @default.
• W2057348102 hasConcept C12554922 @default.
• W2057348102 hasConcept C135983454 @default.
• W2057348102 hasConcept C142613039 @default.
• W2057348102 hasConcept C150194340 @default.
• W2057348102 hasConcept C150903083 @default.
• W2057348102 hasConcept C15152581 @default.
• W2057348102 hasConcept C153911025 @default.
• W2057348102 hasConcept C185592680 @default.
• W2057348102 hasConcept C207001950 @default.
• W2057348102 hasConcept C2779178360 @default.
• W2057348102 hasConcept C2780837183 @default.
• W2057348102 hasConcept C32470452 @default.
• W2057348102 hasConcept C40767141 @default.
• W2057348102 hasConcept C553184892 @default.
• W2057348102 hasConcept C55493867 @default.
• W2057348102 hasConcept C86803240 @default.
• W2057348102 hasConcept C95444343 @default.
• W2057348102 hasConceptScore W2057348102C102230213 @default.
• W2057348102 hasConceptScore W2057348102C104317684 @default.
• W2057348102 hasConceptScore W2057348102C105702510 @default.
• W2057348102 hasConceptScore W2057348102C111599444 @default.
• W2057348102 hasConceptScore W2057348102C12554922 @default.
• W2057348102 hasConceptScore W2057348102C135983454 @default.
• W2057348102 hasConceptScore W2057348102C142613039 @default.
• W2057348102 hasConceptScore W2057348102C150194340 @default.
• W2057348102 hasConceptScore W2057348102C150903083 @default.
• W2057348102 hasConceptScore W2057348102C15152581 @default.
• W2057348102 hasConceptScore W2057348102C153911025 @default.
• W2057348102 hasConceptScore W2057348102C185592680 @default.
• W2057348102 hasConceptScore W2057348102C207001950 @default.
• W2057348102 hasConceptScore W2057348102C2779178360 @default.
• W2057348102 hasConceptScore W2057348102C2780837183 @default.
• W2057348102 hasConceptScore W2057348102C32470452 @default.
• W2057348102 hasConceptScore W2057348102C40767141 @default.

• next