Matches in SemOpenAlex for { <https://semopenalex.org/work/W2057352657> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W2057352657 endingPage "490a" @default.
- W2057352657 startingPage "489a" @default.
- W2057352657 abstract "NMDA receptors are heteromeric glutamate-activated ion channels composed of NR1- and NR2-subunits. Controlled expression of four NR2-isoforms (A - D) results in receptors with distinct gating properties, contributing to the diversity of excitatory post-synaptic currents. Additionally, the NR1/NR2A-isoform can itself respond with distinct kinetics due to modal gating. To investigate whether the NR1/NR2B-isoform also gates with modal kinetics, we recorded steady-state single-channel activity from cell-attached patches of HEK293-cells transfected with NR1 and NR2B, in the continuous presence of saturating agonists. Single-channel records (n=37) revealed a variety of gating patterns illustrated by a 25-fold range in measured equilibrium open probability: range, 0.02 - 0.49; Po (mean ± s.e.m.) = 0.20 ± 0.02. This diversity reflects mainly differences in mean closure durations per file: range, 6 - 200 ms; mean closed time = 43 ± 8 ms; with less spread for mean open durations: range, 1.8 - 10.5 ms; mean open time = 4.8 ± 0.3 ms. Kinetic analyses revealed that each record had 2-4 open and at least 5 closed components in the respective interval duration distributions. As with NR1/2A-receptors, in all NR1/2B-records we observed sporadic gating changes due to sudden changes in mean open durations, indicative of modal behavior. We identified three gating regimes, each having at least two open time components: a ubiquitous brief component (0.27 ± 0.01 ms) and at least one of three longer components (tau-L = 2.5 ± 0.1 ms; tau-M = 5.0 ± 0.2 ms; or tau-H = 10.0 ± 1.0 ms). In contrast to NR1/2A-channel behavior where modal gating allowed characterization of all observed channels, for the NR1/2B-receptor we also observed gating patterns which differ in mean duration of closures. These data and analyses reveal the variety of mechanisms generating the previously observed diversity of macroscopic NR1/2B-responses." @default.
- W2057352657 created "2016-06-24" @default.
- W2057352657 creator A5008685938 @default.
- W2057352657 creator A5017763637 @default.
- W2057352657 creator A5040497202 @default.
- W2057352657 date "2009-02-01" @default.
- W2057352657 modified "2023-09-28" @default.
- W2057352657 title "Diversity of NR1/NR2B Receptor Gating Kinetics" @default.
- W2057352657 doi "https://doi.org/10.1016/j.bpj.2008.12.2528" @default.
- W2057352657 hasPublicationYear "2009" @default.
- W2057352657 type Work @default.
- W2057352657 sameAs 2057352657 @default.
- W2057352657 citedByCount "0" @default.
- W2057352657 crossrefType "journal-article" @default.
- W2057352657 hasAuthorship W2057352657A5008685938 @default.
- W2057352657 hasAuthorship W2057352657A5017763637 @default.
- W2057352657 hasAuthorship W2057352657A5040497202 @default.
- W2057352657 hasBestOaLocation W20573526571 @default.
- W2057352657 hasConcept C104317684 @default.
- W2057352657 hasConcept C121332964 @default.
- W2057352657 hasConcept C12554922 @default.
- W2057352657 hasConcept C148898269 @default.
- W2057352657 hasConcept C170493617 @default.
- W2057352657 hasConcept C185592680 @default.
- W2057352657 hasConcept C194544171 @default.
- W2057352657 hasConcept C50254741 @default.
- W2057352657 hasConcept C53345823 @default.
- W2057352657 hasConcept C55493867 @default.
- W2057352657 hasConcept C61174792 @default.
- W2057352657 hasConcept C62520636 @default.
- W2057352657 hasConcept C86803240 @default.
- W2057352657 hasConceptScore W2057352657C104317684 @default.
- W2057352657 hasConceptScore W2057352657C121332964 @default.
- W2057352657 hasConceptScore W2057352657C12554922 @default.
- W2057352657 hasConceptScore W2057352657C148898269 @default.
- W2057352657 hasConceptScore W2057352657C170493617 @default.
- W2057352657 hasConceptScore W2057352657C185592680 @default.
- W2057352657 hasConceptScore W2057352657C194544171 @default.
- W2057352657 hasConceptScore W2057352657C50254741 @default.
- W2057352657 hasConceptScore W2057352657C53345823 @default.
- W2057352657 hasConceptScore W2057352657C55493867 @default.
- W2057352657 hasConceptScore W2057352657C61174792 @default.
- W2057352657 hasConceptScore W2057352657C62520636 @default.
- W2057352657 hasConceptScore W2057352657C86803240 @default.
- W2057352657 hasIssue "3" @default.
- W2057352657 hasLocation W20573526571 @default.
- W2057352657 hasOpenAccess W2057352657 @default.
- W2057352657 hasPrimaryLocation W20573526571 @default.
- W2057352657 hasRelatedWork W1636088266 @default.
- W2057352657 hasRelatedWork W1980090229 @default.
- W2057352657 hasRelatedWork W2032038036 @default.
- W2057352657 hasRelatedWork W2075494942 @default.
- W2057352657 hasRelatedWork W2097854476 @default.
- W2057352657 hasRelatedWork W2152738980 @default.
- W2057352657 hasRelatedWork W2909181336 @default.
- W2057352657 hasRelatedWork W3001537456 @default.
- W2057352657 hasRelatedWork W3015678452 @default.
- W2057352657 hasRelatedWork W3081261611 @default.
- W2057352657 hasVolume "96" @default.
- W2057352657 isParatext "false" @default.
- W2057352657 isRetracted "false" @default.
- W2057352657 magId "2057352657" @default.
- W2057352657 workType "article" @default.