FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2057365841> ?p ?o ?g. }

• W2057365841 endingPage "662" @default.
• W2057365841 startingPage "655" @default.
• W2057365841 abstract "The cell growth and monoclonal antibody production of the 55-6 hybridoma cell co-cultured with the murine thymoma cell line EL-4 at different initial 55-6:EL-4 ratios were investigated. Both populations were seeded in co-culture without previous stimulation and therefore with low constitutive CD40 and CD40 ligand (CD154) expression levels, and in the absence of exogenous co-stimuli. Viable cell density and growth rate data seem to suggest a competition for nutrients, which is detrimental for both cells in terms of biomass production and also of growth rate for 55-6. Final concentrations of antibody and specific antibody production rates were affected by the initial 55-6:EL-4 ratio. The 4:1 ratio yielded the highest IgG2a concentration, whereas the highest specific antibody production rate was obtained at the 2:1 ratio. Changes mainly in CD154 and also in CD40 expression in co-cultures could suggest cross-talk between both populations. In conclusion, different types of interactions are probably present in this co-culture system: competition for nutrients, cognate interaction and/or autocrine or paracrine interactions that influence the proliferation of both cells and the hybridoma antibody secretion. We are hereby presenting a pre-scale-up process that could speed up the optimization of large-scale monoclonal antibodies production in bioreactors by emulating the in vivo cell–cell interaction between B and T cells without previous stimulation or the addition of co-stimulatory molecules." @default.
• W2057365841 created "2016-06-24" @default.
• W2057365841 creator A5014510070 @default.
• W2057365841 creator A5052197938 @default.
• W2057365841 creator A5072730938 @default.
• W2057365841 creator A5076297661 @default.
• W2057365841 creator A5079449902 @default.
• W2057365841 date "2013-06-14" @default.
• W2057365841 modified "2023-10-17" @default.
• W2057365841 title "Co-culture of the 55-6 B cell hybridoma with the EL-4 thymoma cell. Effect on cell growth and monoclonal antibody production" @default.
• W2057365841 cites W1524553046 @default.
• W2057365841 cites W1653117510 @default.
• W2057365841 cites W1972603102 @default.
• W2057365841 cites W1979034554 @default.
• W2057365841 cites W1979876556 @default.
• W2057365841 cites W1980780414 @default.
• W2057365841 cites W1983182864 @default.
• W2057365841 cites W1993221898 @default.
• W2057365841 cites W2001944699 @default.
• W2057365841 cites W2010147335 @default.
• W2057365841 cites W2034125435 @default.
• W2057365841 cites W2037240183 @default.
• W2057365841 cites W2054814854 @default.
• W2057365841 cites W2060834298 @default.
• W2057365841 cites W2061644646 @default.
• W2057365841 cites W2070720378 @default.
• W2057365841 cites W2078611252 @default.
• W2057365841 cites W2082249681 @default.
• W2057365841 cites W2109617584 @default.
• W2057365841 cites W2110279489 @default.
• W2057365841 cites W2114480393 @default.
• W2057365841 cites W2129551966 @default.
• W2057365841 cites W2148083116 @default.
• W2057365841 cites W2153123202 @default.
• W2057365841 cites W2317559737 @default.
• W2057365841 cites W4256103963 @default.
• W2057365841 doi "https://doi.org/10.1007/s10616-013-9593-z" @default.
• W2057365841 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3720977" @default.
• W2057365841 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23765215" @default.
• W2057365841 hasPublicationYear "2013" @default.
• W2057365841 type Work @default.
• W2057365841 sameAs 2057365841 @default.
• W2057365841 citedByCount "1" @default.
• W2057365841 countsByYear W20573658412015 @default.
• W2057365841 crossrefType "journal-article" @default.
• W2057365841 hasAuthorship W2057365841A5014510070 @default.
• W2057365841 hasAuthorship W2057365841A5052197938 @default.
• W2057365841 hasAuthorship W2057365841A5072730938 @default.
• W2057365841 hasAuthorship W2057365841A5076297661 @default.
• W2057365841 hasAuthorship W2057365841A5079449902 @default.
• W2057365841 hasBestOaLocation W20573658412 @default.
• W2057365841 hasConcept C128240485 @default.
• W2057365841 hasConcept C1491633281 @default.
• W2057365841 hasConcept C153911025 @default.
• W2057365841 hasConcept C154317977 @default.
• W2057365841 hasConcept C159654299 @default.
• W2057365841 hasConcept C170493617 @default.
• W2057365841 hasConcept C202751555 @default.
• W2057365841 hasConcept C203014093 @default.
• W2057365841 hasConcept C2779234475 @default.
• W2057365841 hasConcept C39347974 @default.
• W2057365841 hasConcept C542903549 @default.
• W2057365841 hasConcept C54355233 @default.
• W2057365841 hasConcept C55493867 @default.
• W2057365841 hasConcept C62112901 @default.
• W2057365841 hasConcept C7876069 @default.
• W2057365841 hasConcept C81885089 @default.
• W2057365841 hasConcept C86803240 @default.
• W2057365841 hasConcept C95444343 @default.
• W2057365841 hasConceptScore W2057365841C128240485 @default.
• W2057365841 hasConceptScore W2057365841C1491633281 @default.
• W2057365841 hasConceptScore W2057365841C153911025 @default.
• W2057365841 hasConceptScore W2057365841C154317977 @default.
• W2057365841 hasConceptScore W2057365841C159654299 @default.
• W2057365841 hasConceptScore W2057365841C170493617 @default.
• W2057365841 hasConceptScore W2057365841C202751555 @default.
• W2057365841 hasConceptScore W2057365841C203014093 @default.
• W2057365841 hasConceptScore W2057365841C2779234475 @default.
• W2057365841 hasConceptScore W2057365841C39347974 @default.
• W2057365841 hasConceptScore W2057365841C542903549 @default.
• W2057365841 hasConceptScore W2057365841C54355233 @default.
• W2057365841 hasConceptScore W2057365841C55493867 @default.
• W2057365841 hasConceptScore W2057365841C62112901 @default.
• W2057365841 hasConceptScore W2057365841C7876069 @default.
• W2057365841 hasConceptScore W2057365841C81885089 @default.
• W2057365841 hasConceptScore W2057365841C86803240 @default.
• W2057365841 hasConceptScore W2057365841C95444343 @default.
• W2057365841 hasIssue "4" @default.
• W2057365841 hasLocation W20573658411 @default.
• W2057365841 hasLocation W20573658412 @default.
• W2057365841 hasLocation W20573658413 @default.
• W2057365841 hasLocation W20573658414 @default.
• W2057365841 hasOpenAccess W2057365841 @default.
• W2057365841 hasPrimaryLocation W20573658411 @default.
• W2057365841 hasRelatedWork W1653117510 @default.
• W2057365841 hasRelatedWork W1965454525 @default.
• W2057365841 hasRelatedWork W1991183471 @default.
• W2057365841 hasRelatedWork W2030878664 @default.

• next