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- W2057366309 abstract "Using Saccharomyces cerevisiae as experimental model, we observed that cells mutated in the GTT 1 or GTT 2 genes showed twice as much cadmium absorption than the control strain. We proposed that the formation of the cadmium–glutathione complex is dependent on that transferase, since it was previously demonstrated that the cytoplasmic levels of this complex affect cadmium uptake. The addition of glutathione monoethyl ester (GME), a drug that mimics glutathione (GSH), to gtt 1Δ cells restored the levels of metal absorption to those of the control strain. However, with respect to gtt 2Δ cells, addition of GME did not alter the capacity of removing cadmium from the medium. Taken together, these results suggest that Gtt1 and Gtt2 play different roles in the mechanism of cadmium detoxification. By analyzing the toxic effect of this metal, we verified that gtt 2Δ and gsh 1Δ cells showed, respectively, higher and lower tolerance to cadmium stress than control cells, suggesting that although GSH plays a relevant role in cell protection, formation of the GSH–Cd conjugate is deleterious to the mechanism of defense." @default.
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- W2057366309 date "2004-12-01" @default.
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- W2057366309 title "The role of glutathione transferases in cadmium stress" @default.
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- W2057366309 doi "https://doi.org/10.1016/j.toxlet.2004.07.003" @default.
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