Matches in SemOpenAlex for { <https://semopenalex.org/work/W2057392671> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2057392671 endingPage "4416" @default.
- W2057392671 startingPage "4407" @default.
- W2057392671 abstract "A new human 95 kDa high density lipoprotein (HDL)-binding protein (HBP) corresponding to a high affinity HDL-binding site with K(d) = 1.67 microg/mL and a capacity of 13.4 ng/mg was identified in human fetal hepatocytes. The HDL binding with the 95 kDa HBP plateaus at 2.5-5 microg/mL under reducing and nonreducing conditions. The association of HDL(3) with the 95 kDa HBP plateaued in 15-30 min while dissociation was complete in 30 min. HDL(3), apoA-I, and apoA-II were recognized by the 95 kDa HBP while low density lipoproteins (LDL) and tetranitromethane-modified HDL were not. The 95 kDa HBP predominantly resides on the surface of cells since trypsin treatment of HepG2 cells eliminated nearly 70% of HDL binding. All studied human cells and cell lines (HepG2, Caco-2, HeLa, fibroblasts, SKOV-3, PA-I) demonstrated the presence of the 95 kDa protein. Both RT-PCR and Western blotting for HB-2/ALCAM were negative in human fetal hepatocytes while Gp96/GRP94 was clearly differentiated from the 95 kDa HBP by two-dimensional electrophoretic mobility. Moreover, deglycosylation of HepG2 membrane preparations did not affect either HDL binding to the 95 kDa HBP or its size, while in contrast it affected the molecular weights of HB-2/ALCAM and SR-BI/CLA-1. We conclude that the 95 kDa HBP is a new HDL receptor candidate widely expressed in human cells and cell lines." @default.
- W2057392671 created "2016-06-24" @default.
- W2057392671 creator A5011127352 @default.
- W2057392671 creator A5064197056 @default.
- W2057392671 creator A5081642385 @default.
- W2057392671 creator A5090620149 @default.
- W2057392671 creator A5091269592 @default.
- W2057392671 date "2001-03-15" @default.
- W2057392671 modified "2023-10-18" @default.
- W2057392671 title "Characterization of a 95 kDa High Affinity Human High Density Lipoprotein-Binding Protein†" @default.
- W2057392671 cites W1504706920 @default.
- W2057392671 cites W1574380753 @default.
- W2057392671 cites W1596816338 @default.
- W2057392671 cites W1606423922 @default.
- W2057392671 cites W1997678048 @default.
- W2057392671 cites W2059685472 @default.
- W2057392671 cites W2069185596 @default.
- W2057392671 cites W2072482644 @default.
- W2057392671 cites W2101433730 @default.
- W2057392671 cites W2113504642 @default.
- W2057392671 cites W2166648967 @default.
- W2057392671 doi "https://doi.org/10.1021/bi001503k" @default.
- W2057392671 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11284697" @default.
- W2057392671 hasPublicationYear "2001" @default.
- W2057392671 type Work @default.
- W2057392671 sameAs 2057392671 @default.
- W2057392671 citedByCount "14" @default.
- W2057392671 countsByYear W20573926712015 @default.
- W2057392671 crossrefType "journal-article" @default.
- W2057392671 hasAuthorship W2057392671A5011127352 @default.
- W2057392671 hasAuthorship W2057392671A5064197056 @default.
- W2057392671 hasAuthorship W2057392671A5081642385 @default.
- W2057392671 hasAuthorship W2057392671A5090620149 @default.
- W2057392671 hasAuthorship W2057392671A5091269592 @default.
- W2057392671 hasConcept C104317684 @default.
- W2057392671 hasConcept C1491633281 @default.
- W2057392671 hasConcept C153911025 @default.
- W2057392671 hasConcept C181199279 @default.
- W2057392671 hasConcept C185592680 @default.
- W2057392671 hasConcept C2778163477 @default.
- W2057392671 hasConcept C2780072125 @default.
- W2057392671 hasConcept C2780340462 @default.
- W2057392671 hasConcept C2780456743 @default.
- W2057392671 hasConcept C2780541478 @default.
- W2057392671 hasConcept C54355233 @default.
- W2057392671 hasConcept C553089730 @default.
- W2057392671 hasConcept C55493867 @default.
- W2057392671 hasConcept C58121356 @default.
- W2057392671 hasConcept C81885089 @default.
- W2057392671 hasConcept C85265743 @default.
- W2057392671 hasConcept C86803240 @default.
- W2057392671 hasConceptScore W2057392671C104317684 @default.
- W2057392671 hasConceptScore W2057392671C1491633281 @default.
- W2057392671 hasConceptScore W2057392671C153911025 @default.
- W2057392671 hasConceptScore W2057392671C181199279 @default.
- W2057392671 hasConceptScore W2057392671C185592680 @default.
- W2057392671 hasConceptScore W2057392671C2778163477 @default.
- W2057392671 hasConceptScore W2057392671C2780072125 @default.
- W2057392671 hasConceptScore W2057392671C2780340462 @default.
- W2057392671 hasConceptScore W2057392671C2780456743 @default.
- W2057392671 hasConceptScore W2057392671C2780541478 @default.
- W2057392671 hasConceptScore W2057392671C54355233 @default.
- W2057392671 hasConceptScore W2057392671C553089730 @default.
- W2057392671 hasConceptScore W2057392671C55493867 @default.
- W2057392671 hasConceptScore W2057392671C58121356 @default.
- W2057392671 hasConceptScore W2057392671C81885089 @default.
- W2057392671 hasConceptScore W2057392671C85265743 @default.
- W2057392671 hasConceptScore W2057392671C86803240 @default.
- W2057392671 hasIssue "14" @default.
- W2057392671 hasLocation W20573926711 @default.
- W2057392671 hasLocation W20573926712 @default.
- W2057392671 hasOpenAccess W2057392671 @default.
- W2057392671 hasPrimaryLocation W20573926711 @default.
- W2057392671 hasRelatedWork W1975290430 @default.
- W2057392671 hasRelatedWork W2006947702 @default.
- W2057392671 hasRelatedWork W2077658731 @default.
- W2057392671 hasRelatedWork W2142633849 @default.
- W2057392671 hasRelatedWork W2259771725 @default.
- W2057392671 hasRelatedWork W2352271508 @default.
- W2057392671 hasRelatedWork W2417380264 @default.
- W2057392671 hasRelatedWork W2420818573 @default.
- W2057392671 hasRelatedWork W3148670365 @default.
- W2057392671 hasRelatedWork W4250139314 @default.
- W2057392671 hasVolume "40" @default.
- W2057392671 isParatext "false" @default.
- W2057392671 isRetracted "false" @default.
- W2057392671 magId "2057392671" @default.
- W2057392671 workType "article" @default.