Matches in SemOpenAlex for { <https://semopenalex.org/work/W2057458567> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2057458567 endingPage "4262" @default.
- W2057458567 startingPage "4255" @default.
- W2057458567 abstract "Dendritic cells (DCs), instructed by the priming signals from microbial factors, can produce interleukin (IL)- 12p70 and promote T helper (Th)1 proliferation and interferon (IFN)-γ production. This event seems to be critical for the containment of infections caused by intracellular pathogens, even including Leishmania infection. In the present in vitro study we have investigated: 1) phagocytic capacities and IL-12 production by human monocyte-derived DCs and macrophages (Mφs), infected with Leishmania infantum promastigotes; 2) IFN-γ production by human CD4+ T cells coincubated with DCs or macrophages pulsed with live promastigotes. Monocyte-derived myeloid DCs and Mφs from healthy donors were infected with live metacyclic Leishmania infantum (MON-1) promastigotes, previously opsonized with 5% autologous serum, at 1:4 cell/parasite ratio. Percentage and index of phagocytosis were calculated after 2, 24 and 48 h of incubation. IL-12 production was evaluated by an ELISA in supernatants from 48 h Leishmania-infected or lipopolysaccharides (LPS)-stimulated DCs and Mφs, also in the presence of phytohemagglutinin-activated or inactivated CD4+ T cells. For IFN-γ production, CD4+ T cells were repeatedly stimulated with DCs or Mφs, pulsed with live Leishmania promastigotes or activated with LPS. The number of IFN-γ-secreting cells was evaluated by an ELISpot assay. Results showed that Mφs have a higher phagocytic capacity towards L. infantum promastigotes than DCs. Moreover, unlike Mφs, Leishmania-infected DCs were able to release IL-12p70; this production significantly increased in the presence of activated CD4+ T cells. Finally, DCs pulsed with live parasites and added to autologous CD4+ T cells induced a higher number of IFN- γ-secreting cells than Mφs, thus indicating their ability to polarize Th cells toward the Th1 subset. These data indicate that DCs are able to promote protective Th1 immune responses in our experimental model of Leishmania infantum infection, thus representing the grounds for initiating immunoterapeutic and vaccinal strategies. Keywords: Leishmania, IL-12, phagocytosis, Lymphocytes, interferon" @default.
- W2057458567 created "2016-06-24" @default.
- W2057458567 creator A5001338635 @default.
- W2057458567 creator A5006947722 @default.
- W2057458567 creator A5038081452 @default.
- W2057458567 creator A5049166129 @default.
- W2057458567 creator A5056559176 @default.
- W2057458567 creator A5058465993 @default.
- W2057458567 creator A5062475284 @default.
- W2057458567 creator A5069188297 @default.
- W2057458567 creator A5086897295 @default.
- W2057458567 date "2006-11-01" @default.
- W2057458567 modified "2023-09-25" @default.
- W2057458567 title "Toll-Like Receptor-Positive Cells and Recognition of Pathogens: How Human Myeloid Dendritic Cells Respond to In Vitro Infection with Leishmania infantum" @default.
- W2057458567 doi "https://doi.org/10.2174/138161206778743556" @default.
- W2057458567 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17100627" @default.
- W2057458567 hasPublicationYear "2006" @default.
- W2057458567 type Work @default.
- W2057458567 sameAs 2057458567 @default.
- W2057458567 citedByCount "6" @default.
- W2057458567 countsByYear W20574585672012 @default.
- W2057458567 countsByYear W20574585672013 @default.
- W2057458567 countsByYear W20574585672016 @default.
- W2057458567 crossrefType "journal-article" @default.
- W2057458567 hasAuthorship W2057458567A5001338635 @default.
- W2057458567 hasAuthorship W2057458567A5006947722 @default.
- W2057458567 hasAuthorship W2057458567A5038081452 @default.
- W2057458567 hasAuthorship W2057458567A5049166129 @default.
- W2057458567 hasAuthorship W2057458567A5056559176 @default.
- W2057458567 hasAuthorship W2057458567A5058465993 @default.
- W2057458567 hasAuthorship W2057458567A5062475284 @default.
- W2057458567 hasAuthorship W2057458567A5069188297 @default.
- W2057458567 hasAuthorship W2057458567A5086897295 @default.
- W2057458567 hasConcept C136764020 @default.
- W2057458567 hasConcept C149532602 @default.
- W2057458567 hasConcept C154317977 @default.
- W2057458567 hasConcept C160448771 @default.
- W2057458567 hasConcept C187316574 @default.
- W2057458567 hasConcept C202751555 @default.
- W2057458567 hasConcept C203014093 @default.
- W2057458567 hasConcept C2776555147 @default.
- W2057458567 hasConcept C2778689377 @default.
- W2057458567 hasConcept C2780599195 @default.
- W2057458567 hasConcept C2781092759 @default.
- W2057458567 hasConcept C2781257258 @default.
- W2057458567 hasConcept C41008148 @default.
- W2057458567 hasConcept C55493867 @default.
- W2057458567 hasConcept C71928629 @default.
- W2057458567 hasConcept C86803240 @default.
- W2057458567 hasConcept C8891405 @default.
- W2057458567 hasConcept C89423630 @default.
- W2057458567 hasConceptScore W2057458567C136764020 @default.
- W2057458567 hasConceptScore W2057458567C149532602 @default.
- W2057458567 hasConceptScore W2057458567C154317977 @default.
- W2057458567 hasConceptScore W2057458567C160448771 @default.
- W2057458567 hasConceptScore W2057458567C187316574 @default.
- W2057458567 hasConceptScore W2057458567C202751555 @default.
- W2057458567 hasConceptScore W2057458567C203014093 @default.
- W2057458567 hasConceptScore W2057458567C2776555147 @default.
- W2057458567 hasConceptScore W2057458567C2778689377 @default.
- W2057458567 hasConceptScore W2057458567C2780599195 @default.
- W2057458567 hasConceptScore W2057458567C2781092759 @default.
- W2057458567 hasConceptScore W2057458567C2781257258 @default.
- W2057458567 hasConceptScore W2057458567C41008148 @default.
- W2057458567 hasConceptScore W2057458567C55493867 @default.
- W2057458567 hasConceptScore W2057458567C71928629 @default.
- W2057458567 hasConceptScore W2057458567C86803240 @default.
- W2057458567 hasConceptScore W2057458567C8891405 @default.
- W2057458567 hasConceptScore W2057458567C89423630 @default.
- W2057458567 hasIssue "32" @default.
- W2057458567 hasLocation W20574585671 @default.
- W2057458567 hasLocation W20574585672 @default.
- W2057458567 hasOpenAccess W2057458567 @default.
- W2057458567 hasPrimaryLocation W20574585671 @default.
- W2057458567 hasRelatedWork W1991258842 @default.
- W2057458567 hasRelatedWork W1997570781 @default.
- W2057458567 hasRelatedWork W2024431897 @default.
- W2057458567 hasRelatedWork W2029588651 @default.
- W2057458567 hasRelatedWork W2052491883 @default.
- W2057458567 hasRelatedWork W2089717661 @default.
- W2057458567 hasRelatedWork W2420906302 @default.
- W2057458567 hasRelatedWork W2948640242 @default.
- W2057458567 hasRelatedWork W3034783730 @default.
- W2057458567 hasRelatedWork W4313374704 @default.
- W2057458567 hasVolume "12" @default.
- W2057458567 isParatext "false" @default.
- W2057458567 isRetracted "false" @default.
- W2057458567 magId "2057458567" @default.
- W2057458567 workType "article" @default.