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- W2057476088 abstract "Milk IgA was seen to be largely associated with milk fat globules (13–23 of the sIgA in whole milk). Isolated fat globules became agglutinated by anti-IgA and to a lesser degree by anti-IgM but not by anti-IgG1 or anti-IgG2 (microscopic plate agglutination). Absorption studies using anti-bovine sIgA showed that the cream contained some 16 times more IgA than the repective whey. Isolated milk fat globule membranes released IgA and SC when treated by Triton X 100 as demonstrated by double gel diffusion. IgA was the only membrane-associated component showing cross-reactivity with serum proteins in immunoelectrophoresis. An antiserum prepared against fat globule membranes showed strong anti-IgA activity. IgA from whey became absorbed into washed cream. The specificity of MFGM associated immunity was demonstrated by analysing the capacity of cream, isolated from mastitic cows, to bind homologous labelled bacteria (cream-rising test). Les IgA du lait semblent être largement associées aux globules gras (13–23 des IgA du lait). Les globules gras isolés agglutinent avec un serum anti-IgA d'un degré moindre avec un serum anti IgM et pas du tout avec des serums anti IgG1 ou IgG2 (agglutination microscopique sur lame). L'étude de l'absorption utilisant le serum bovin anti IgA montre que la crème contient 16 fois plus d'IgA que le petit lait. La membrane du globule gras isolé du lait (MFGM) libère après traitement par le Triton X 100 des IgA et des pièces sécrétoires mises en évidence par une double diffusion sur gel. L'IgA est le seul composant associé à la membrane qui présente des reactions croisées avec un serum proteique par electrophorèse. Un antiserum préparé à partir de la membrane d'un globule gras montre une activité importante anti IgA. La spécificité de l'immunité associé aux MFGM a été démontrée en analysant la capacité de la crème, isolée de lait de mammite bovine, à fixer les bacteries marquées homologues (cream-rising test)." @default.
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- W2057476088 date "1981-01-01" @default.
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- W2057476088 title "Association of bovine secretory immunoglobulins with milk fat globule membranes" @default.
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- W2057476088 doi "https://doi.org/10.1016/0147-9571(81)90019-9" @default.
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