FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2057497441> ?p ?o ?g. }

• W2057497441 endingPage "1074" @default.
• W2057497441 startingPage "1074" @default.
• W2057497441 abstract "Potato (Solanum tuberosum L.) is widely grown as a staple food and cash crop in Tajikistan and is an important food security crop in the country. In June 2011, we conducted a survey of potatoes in farmers' fields in the Buston and Dushanbe regions (about 200 miles apart) of Tajikistan. Potato plants with stunted growth and leaves showing chlorotic spots, curling, and necrotic spots and rings were observed with the disease incidence monitored in 10 fields each in Buston and Dushanbe areas varying between 10 and 60%. Representative samples from symptomatic plants tested positive for Potato virus Y (PVY) using virus-specific immunostrips (Agdia Inc., Elkhart, IN). Leaf samples from symptomatic plants were collected from Buston and Dushanbe areas, imprinted on FTA Classic Cards (Whatman International Ltd., Maidstone, UK), air dried, and shipped to the lab at Washington State University for confirmatory diagnostic tests. Total nucleic acids were eluted from FTA cards (1) and subjected to reverse transcription (RT)-PCR with primers (PVY/Y4A and PVY/Y3S) specific to the coat protein of PVY (3). Samples infected with PVY ordinary strain (PVY O ), tuber necrosis strain (PVY NTN ), tobacco veinal necrosis strains (PVY EU-N and PVY NA-N ), and a recombinant strain (PVY N:O ) were included as references to validate RT-PCR results. A single DNA product of approximately 480 bp was amplified from potato samples that tested positive with PVY-specific immunostrips. The amplified fragments from two samples from Dushanbe and six from Buston areas were cloned separately into pCR2.1 (Invitrogen Corp., Carlsbad, CA) and two independent clones per amplicon were sequenced from both orientations. Pairwise comparison of these sequences showed 90 to 100% identity among the cloned amplicons (GenBank Accession Nos. JQ743609 to JQ743616) and 90 to 100% with corresponding nucleotide sequence of reference PVY strains (GenBank Accession Nos. JQ743617 to JQ743621). A global phylogenetic analysis of sequences revealed the presence of PVY O in both samples from Dushanbe and one sample from Buston regions and presence of PVY NTN in the remaining five samples from the Buston region. Because of the possible occurrence of mixed infections of PVY strains (2), further studies are needed to determine the presence of mixed infections of two or more strains of PVY and their specificity to potato cultivars. To our knowledge, this study represents the first confirmed report of two distinct strains of PVY in potato in Tajikistan. The occurrence of PVY NTN , a quarantine pathogen in many countries (2), warrants additional investigations to improve sanitary status of potato fields and to facilitate the availability of virus-free seed in clean plant programs for significant yield increases in Tajikistan. References: (1) O. J. Alabi et al. J. Virol. Methods 154:111, 2008. (2) S. Gray et al. Plant Dis. 94:1384, 2010. (3) R. P. Singh et al. J. Virol. Methods 59:189, 1996." @default.
• W2057497441 created "2016-06-24" @default.
• W2057497441 creator A5017466263 @default.
• W2057497441 creator A5025111262 @default.
• W2057497441 creator A5043490390 @default.
• W2057497441 creator A5073214462 @default.
• W2057497441 date "2012-07-01" @default.
• W2057497441 modified "2023-09-30" @default.
• W2057497441 title "First Report of <i>Potato virus Y</i> in Potato in Tajikistan" @default.
• W2057497441 doi "https://doi.org/10.1094/pdis-03-12-0249-pdn" @default.
• W2057497441 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30727233" @default.
• W2057497441 hasPublicationYear "2012" @default.
• W2057497441 type Work @default.
• W2057497441 sameAs 2057497441 @default.
• W2057497441 citedByCount "4" @default.
• W2057497441 countsByYear W20574974412016 @default.
• W2057497441 countsByYear W20574974412019 @default.
• W2057497441 countsByYear W20574974412022 @default.
• W2057497441 crossrefType "journal-article" @default.
• W2057497441 hasAuthorship W2057497441A5017466263 @default.
• W2057497441 hasAuthorship W2057497441A5025111262 @default.
• W2057497441 hasAuthorship W2057497441A5043490390 @default.
• W2057497441 hasAuthorship W2057497441A5073214462 @default.
• W2057497441 hasBestOaLocation W20574974411 @default.
• W2057497441 hasConcept C109110057 @default.
• W2057497441 hasConcept C133604927 @default.
• W2057497441 hasConcept C137580998 @default.
• W2057497441 hasConcept C144027150 @default.
• W2057497441 hasConcept C159047783 @default.
• W2057497441 hasConcept C2522874641 @default.
• W2057497441 hasConcept C2776625791 @default.
• W2057497441 hasConcept C2780504191 @default.
• W2057497441 hasConcept C2781255879 @default.
• W2057497441 hasConcept C42972112 @default.
• W2057497441 hasConcept C59822182 @default.
• W2057497441 hasConcept C6557445 @default.
• W2057497441 hasConcept C71924100 @default.
• W2057497441 hasConcept C86803240 @default.
• W2057497441 hasConceptScore W2057497441C109110057 @default.
• W2057497441 hasConceptScore W2057497441C133604927 @default.
• W2057497441 hasConceptScore W2057497441C137580998 @default.
• W2057497441 hasConceptScore W2057497441C144027150 @default.
• W2057497441 hasConceptScore W2057497441C159047783 @default.
• W2057497441 hasConceptScore W2057497441C2522874641 @default.
• W2057497441 hasConceptScore W2057497441C2776625791 @default.
• W2057497441 hasConceptScore W2057497441C2780504191 @default.
• W2057497441 hasConceptScore W2057497441C2781255879 @default.
• W2057497441 hasConceptScore W2057497441C42972112 @default.
• W2057497441 hasConceptScore W2057497441C59822182 @default.
• W2057497441 hasConceptScore W2057497441C6557445 @default.
• W2057497441 hasConceptScore W2057497441C71924100 @default.
• W2057497441 hasConceptScore W2057497441C86803240 @default.
• W2057497441 hasIssue "7" @default.
• W2057497441 hasLocation W20574974411 @default.
• W2057497441 hasLocation W20574974412 @default.
• W2057497441 hasOpenAccess W2057497441 @default.
• W2057497441 hasPrimaryLocation W20574974411 @default.
• W2057497441 hasRelatedWork W134032564 @default.
• W2057497441 hasRelatedWork W1600253410 @default.
• W2057497441 hasRelatedWork W2002799306 @default.
• W2057497441 hasRelatedWork W2009207679 @default.
• W2057497441 hasRelatedWork W2061321142 @default.
• W2057497441 hasRelatedWork W2068896267 @default.
• W2057497441 hasRelatedWork W2140405337 @default.
• W2057497441 hasRelatedWork W2262491234 @default.
• W2057497441 hasRelatedWork W2553964630 @default.
• W2057497441 hasRelatedWork W2980547 @default.
• W2057497441 hasVolume "96" @default.
• W2057497441 isParatext "false" @default.
• W2057497441 isRetracted "false" @default.
• W2057497441 magId "2057497441" @default.
• W2057497441 workType "article" @default.