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• W2057516378 startingPage "271" @default.
• W2057516378 abstract "The contact with the postsynaptic target induces structural and functional modifications in the serotonergic cell C1 of Helix pomatia. In previous studies we have found that the presence of a non-physiological target down-regulates the number of presynaptic varicosities formed by cultured C1 neurons and has a strong inhibitory effect on the action potential-evoked Ca(2+) influx and neurotransmitter release at C1 terminals. Since a large body of experimental evidence implicates the synapsins in the development and functional maturation of synaptic connections, we have investigated whether the injection of exogenous synapsin I into the presynaptic neuron C1 could affect the inhibitory effect of the wrong target on neurotransmitter release. C1 neurons were cultured with the wrong target neuron C3 for three to five days and then injected with either dephosphorylated or Ca(2+)/calmodulin-dependent protein kinase II-phosphorylated Cy3-labeled synapsin I. The subcellular distribution of exogenous synapsin I, followed by fluorescence videomicroscopy, revealed that only synapsin I phosphorylated by Ca(2+)/calmodulin-dependent protein kinase II diffused in the cytoplasm and reached the terminal arborizations of the axon, while the dephosphorylated form did not diffuse beyond the cell body. Evoked neurotransmitter release was measured during C1 stimulation using a freshly dissociated neuron B2 (sniffer) micromanipulated in close contact with the terminals of C1. A three-fold increase in the amplitude of the sniffer depolarization with respect to the pre-injection amplitude (190+/-29% increase, n=10, P<0.006) was found 5 min after injection of Ca(2+)/calmodulin-dependent protein kinase II-phosphorylated synapsin I that lasted for about 30 min. No significant change was observed after injection of buffer or dephosphorylated synapsin I. These data indicate that the presence of synapsin I induces a fast increase in neurotransmitter release that overcomes the inhibitory effect of the non-physiological target and suggest that the expression of synapsins may play a role in the modulation of synaptic strength and neural connectivity." @default.
• W2057516378 created "2016-06-24" @default.
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• W2057516378 creator A5073562468 @default.
• W2057516378 creator A5083243484 @default.
• W2057516378 date "2001-04-01" @default.
• W2057516378 modified "2023-09-23" @default.
• W2057516378 title "Intracellular injection of synapsin I induces neurotransmitter release in C1 neurons of Helix pomatia contacting a wrong target" @default.
• W2057516378 cites W1489351324 @default.
• W2057516378 cites W1513601556 @default.
• W2057516378 cites W1527438907 @default.
• W2057516378 cites W1538692246 @default.
• W2057516378 cites W1547416252 @default.
• W2057516378 cites W1599287250 @default.
• W2057516378 cites W1705159546 @default.
• W2057516378 cites W1917414302 @default.
• W2057516378 cites W1934569220 @default.
• W2057516378 cites W1963783599 @default.
• W2057516378 cites W1968396722 @default.
• W2057516378 cites W1968596306 @default.
• W2057516378 cites W1972288588 @default.
• W2057516378 cites W1977473049 @default.
• W2057516378 cites W1982014176 @default.
• W2057516378 cites W1986512833 @default.
• W2057516378 cites W1987040923 @default.
• W2057516378 cites W1990253183 @default.
• W2057516378 cites W1992156395 @default.
• W2057516378 cites W1998232144 @default.
• W2057516378 cites W2000269403 @default.
• W2057516378 cites W2004191360 @default.
• W2057516378 cites W2006420660 @default.
• W2057516378 cites W2008476076 @default.
• W2057516378 cites W2010005988 @default.
• W2057516378 cites W2010313687 @default.
• W2057516378 cites W2011137853 @default.
• W2057516378 cites W2012627635 @default.
• W2057516378 cites W2013907969 @default.
• W2057516378 cites W2016823691 @default.
• W2057516378 cites W2021295015 @default.
• W2057516378 cites W2022046189 @default.
• W2057516378 cites W2022740810 @default.
• W2057516378 cites W2025761398 @default.
• W2057516378 cites W2027361206 @default.
• W2057516378 cites W2029198617 @default.
• W2057516378 cites W2034685321 @default.
• W2057516378 cites W2037490893 @default.
• W2057516378 cites W2038789857 @default.
• W2057516378 cites W2041199542 @default.
• W2057516378 cites W2041936778 @default.
• W2057516378 cites W2044885256 @default.
• W2057516378 cites W2046990869 @default.
• W2057516378 cites W2047143441 @default.
• W2057516378 cites W2054473035 @default.
• W2057516378 cites W2061260576 @default.
• W2057516378 cites W2064119552 @default.
• W2057516378 cites W2064565635 @default.
• W2057516378 cites W2067481095 @default.
• W2057516378 cites W2070171796 @default.
• W2057516378 cites W2071896888 @default.
• W2057516378 cites W2076636373 @default.
• W2057516378 cites W2078889734 @default.
• W2057516378 cites W2082751682 @default.
• W2057516378 cites W2085825273 @default.
• W2057516378 cites W2092137723 @default.
• W2057516378 cites W2100837269 @default.
• W2057516378 cites W2101108802 @default.
• W2057516378 cites W2109400189 @default.
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• W2057516378 cites W2114141470 @default.
• W2057516378 cites W2123557321 @default.
• W2057516378 cites W2130185623 @default.
• W2057516378 cites W2145211070 @default.
• W2057516378 cites W2146947437 @default.
• W2057516378 cites W2153519347 @default.
• W2057516378 cites W2154922728 @default.
• W2057516378 cites W2159895218 @default.
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• W2057516378 doi "https://doi.org/10.1016/s0306-4522(01)00063-x" @default.
• W2057516378 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11311549" @default.
• W2057516378 hasPublicationYear "2001" @default.
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