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- W2057603215 abstract "Non canonical DNA structures are becoming a point of attention, because repetitive sequences have shown to adopt other alternative conformations to B-DNA. Here we show the analysis by electrophoresis and spectroscopic methods of sequences that form what has been named as DNA. These DNA is assembled from two different DNA strands that have a mismatched region that it is believe contains bases stacked rather than regular hydrogen bonding pairing, this stabilization pattern is referred as zipper-like. We used a zipper-like motif of eight G's mismatch base pairs bracked by B-form geometry at its sides. Subsequently, two strands of DNA were added in a preformed buffer with K+ ions which allowed the formation of a quadruplex synapsable via Hoogsteen hydrogen bonding. With Atomic Force Microscopy we found fibers assembled from these sequences. We hypothesize that the stability and assemble mechanism of the structures formed is the result of the guanine quartet that gives greater rigidity compared to DNA double. This type of DNA could be important for matrices and nanotechnology applications." @default.
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- W2057603215 date "2013-01-01" @default.
- W2057603215 modified "2023-09-27" @default.
- W2057603215 title "DNA Assembly of Nanofibers from Duplex - Quadruplex DNA" @default.
- W2057603215 doi "https://doi.org/10.1016/j.bpj.2012.11.1851" @default.
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