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- W2057632215 abstract "Abstract Sperm, manually stripped from male Sillago ciliata , were successfully cryopreserved in the vapour of liquid N 2 using glycerol or DMSO as the cryoprotectant. Methanol and propylene glycol had no cryoprotective effect on the summer whiting sperm. There were no significant differences in post-thaw fertility or duration of motility of the spermatozoa frozen with DMSO or glycerol and glycerol was used for further trials. The post-thaw duration of motility was improved with the addition of egg yolk to the cryoprotectant mixture. The cryoprotectant medium contained 1.25 M glycerol in fish Ringer; egg yolk was added (40% v/v ) (final concentration of 0.645 m M ) and the semen diluted 6.74, 1. Freezing was best performed in liquid nitrogen vapour with transfer and storage in liquid nitrogen; instant immersion in liquid nitrogen reduced the viability of the sperm. Storage in liquid nitrogen over a 7-day period did not significantly affect duration of motility. Equilibration prior to freezing was not necessary and sperm could be thawed at a range of temperatures from 0 ° to 40 °C without affecting their post-thaw duration of motility." @default.
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- W2057632215 date "1992-03-01" @default.
- W2057632215 modified "2023-09-28" @default.
- W2057632215 title "Cryopreservation of summer whiting (Sillago ciliata) spermatozoa" @default.
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- W2057632215 doi "https://doi.org/10.1016/0044-8486(92)90297-x" @default.
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