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- W2057661688 abstract "Neuronal activity causes substantial postsynaptic Na + transients. However, the physiological consequences of such Na + transients remain largely unknown. High-resolution Na + imaging is pivotal to study these questions, and, up to now, two-photon imaging with the fluorescent Na + indicator sodium-binding benzofuran isophthalate (SBFI) has been the method of choice. When introduced into individual neurons in acute tissue slices, SBFI enables measurements of [Na + ] i transients in dendrites and spines. This technique is also suitable for the determination of [Na + ] i transients in other cell types, such as glial cells, with high spatial resolution, and is likely to be useful for measurement of Na + signals in vivo. This protocol provides guidelines and tips for measuring dynamic Na + concentrations within neurons, as well as an in situ calibration procedure. Because the basic properties of SBFI inside a cell differ significantly from the same properties in vitro, in situ calibrations are required to obtain meaningful experimental results." @default.
- W2057661688 created "2016-06-24" @default.
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- W2057661688 date "2012-11-01" @default.
- W2057661688 modified "2023-10-14" @default.
- W2057661688 title "Two-Photon Sodium Imaging in Dendritic Spines" @default.
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- W2057661688 doi "https://doi.org/10.1101/pdb.prot072074" @default.
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