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• W2057673816 startingPage "45" @default.
• W2057673816 abstract "Rad51, Rad52, and replication protein-A (RPA) play crucial roles in the repair of DNA double-strand breaks in Saccharomyces cerevisiae. Rad51 mediates DNA strand exchange, a key reaction in DNA recombination. Rad52 recruits Rad51 into single-stranded DNAs (ssDNAs) that are saturated with RPA. Rad52 also promotes annealing of ssDNA strands that are complexed with RPA. Specific protein-protein interactions are involved in these reactions. Here we report new biochemical characteristics of these protein interactions. First, Rad52-RPA interaction requires multiple molecules of RPA to be associated with ssDNA, suggesting that multiple contacts between the Rad52 ring and RPA-ssDNA filament are needed for stable binding. Second, RPA-t11, which is a recombination-deficient mutant of RPA, displays a defect in interacting with Rad52 in the presence of salt above 50 mM, explaining the defect in Rad52-mediated ssDNA annealing in the presence of this mutation. Third, ssDNA annealing promoted by Rad52 is preceded by aggregation of multiple RPA-ssDNA complexes with Rad52, and Rad51 inhibits this aggregation. These results suggest a regulatory role for Rad51 that suppresses ssDNA annealing and facilitates DNA strand invasion. Finally, the Rad51-double-stranded DNA complex disrupts Rad52-RPA interaction in ssDNA and titrates Rad52 from RPA. This suggests an additional regulatory role for Rad51 following DNA strand invasion, where Rad51-double-stranded DNA may inhibit illegitimate second-end capture to ensure the error-free repair of a DNA double-strand break." @default.
• W2057673816 created "2016-06-24" @default.
• W2057673816 creator A5015339213 @default.
• W2057673816 creator A5061352298 @default.
• W2057673816 date "2009-07-01" @default.
• W2057673816 modified "2023-09-26" @default.
• W2057673816 title "Dynamic Regulatory Interactions of Rad51, Rad52, and Replication Protein-A in Recombination Intermediates" @default.
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• W2057673816 doi "https://doi.org/10.1016/j.jmb.2009.05.009" @default.
• W2057673816 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19445949" @default.
• W2057673816 hasPublicationYear "2009" @default.
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