Matches in SemOpenAlex for { <https://semopenalex.org/work/W2057788907> ?p ?o ?g. }
- W2057788907 endingPage "1980" @default.
- W2057788907 startingPage "1970" @default.
- W2057788907 abstract "Abstract The canonical and noncanonical NFκB signaling pathways regulate a variety of cellular activities; however, their functions in human embryonic stem cells (hESCs) have not been fully investigated. Expression studies during hESC differentiation indicated a significant increase in the expression of two key components of the canonical NFκB pathway (p50 and Ser529 phosphorylated form of p65) as well as a significant reduction in expression of key components of the noncanonical NFκB pathway [v-rel reticuloendotheliosis viral oncogene homolog B (RELB), p52, NIK]. Inhibition of canonical NFκB resulted in hESC apoptosis, changes in cell cycle distribution, and reduced hESC proliferation. In addition, inhibition of canonical NFκB was associated with significant changes in NANOG and OCT4 expression, suppression of differentiation toward all primitive extraembryonic and embryonic lineages with the exception of primitive ectoderm and ectodermal lineages. Inhibition of noncanonical NFκB via small interfering RNA-mediated downregulation of RELB resulted in reduced hESC proliferation and opposite changes to expression of key differentiation lineage markers genes when compared with downregulation of canonical NF-κB. Chromatin immunoprecipitation assays indicated binding of p65 and RELB to regulatory regions of key differentiation marker genes suggesting a direct transcriptional role for both branches of this pathway in hESC. These findings coupled with opposing trends in expression of key components during hESC differentiation, suggests a fine and opposing balance between the two branches of NFκB signaling pathways and their involvement in two distinct processes: the canonical pathway regulating hESC differentiation and the noncanonical pathway maintaining hESC pluripotency." @default.
- W2057788907 created "2016-06-24" @default.
- W2057788907 creator A5006777495 @default.
- W2057788907 creator A5020825003 @default.
- W2057788907 creator A5032539966 @default.
- W2057788907 creator A5038567260 @default.
- W2057788907 creator A5045546704 @default.
- W2057788907 creator A5064384359 @default.
- W2057788907 creator A5085260473 @default.
- W2057788907 date "2010-11-01" @default.
- W2057788907 modified "2023-10-16" @default.
- W2057788907 title "Opposing Putative Roles for Canonical and Noncanonical NFκB Signaling on the Survival, Proliferation, and Differentiation Potential of Human Embryonic Stem Cells" @default.
- W2057788907 cites W1533671266 @default.
- W2057788907 cites W1661180389 @default.
- W2057788907 cites W1767114057 @default.
- W2057788907 cites W1868273134 @default.
- W2057788907 cites W1880841984 @default.
- W2057788907 cites W1963549994 @default.
- W2057788907 cites W1963749512 @default.
- W2057788907 cites W1968013945 @default.
- W2057788907 cites W1971459774 @default.
- W2057788907 cites W1973862406 @default.
- W2057788907 cites W1977348907 @default.
- W2057788907 cites W1977911088 @default.
- W2057788907 cites W1986301169 @default.
- W2057788907 cites W1987097007 @default.
- W2057788907 cites W1988463809 @default.
- W2057788907 cites W1999982643 @default.
- W2057788907 cites W2004972310 @default.
- W2057788907 cites W2005025375 @default.
- W2057788907 cites W2005470498 @default.
- W2057788907 cites W2008856081 @default.
- W2057788907 cites W2013731535 @default.
- W2057788907 cites W2023500065 @default.
- W2057788907 cites W2029859452 @default.
- W2057788907 cites W2031495220 @default.
- W2057788907 cites W2036596287 @default.
- W2057788907 cites W2038975875 @default.
- W2057788907 cites W2039388529 @default.
- W2057788907 cites W2047051710 @default.
- W2057788907 cites W2047860183 @default.
- W2057788907 cites W2050265341 @default.
- W2057788907 cites W2051831012 @default.
- W2057788907 cites W2053111401 @default.
- W2057788907 cites W2066868766 @default.
- W2057788907 cites W2074150439 @default.
- W2057788907 cites W2091541206 @default.
- W2057788907 cites W2096589599 @default.
- W2057788907 cites W2106278040 @default.
- W2057788907 cites W2123759290 @default.
- W2057788907 cites W2133233551 @default.
- W2057788907 cites W2135974887 @default.
- W2057788907 cites W2138580291 @default.
- W2057788907 cites W2138804143 @default.
- W2057788907 cites W2148119611 @default.
- W2057788907 cites W2148380416 @default.
- W2057788907 cites W2151166716 @default.
- W2057788907 cites W2159219386 @default.
- W2057788907 cites W2160077902 @default.
- W2057788907 cites W2167548363 @default.
- W2057788907 cites W2315014732 @default.
- W2057788907 cites W2999065750 @default.
- W2057788907 cites W4247033596 @default.
- W2057788907 cites W4379365691 @default.
- W2057788907 doi "https://doi.org/10.1002/stem.528" @default.
- W2057788907 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20882529" @default.
- W2057788907 hasPublicationYear "2010" @default.
- W2057788907 type Work @default.
- W2057788907 sameAs 2057788907 @default.
- W2057788907 citedByCount "34" @default.
- W2057788907 countsByYear W20577889072012 @default.
- W2057788907 countsByYear W20577889072013 @default.
- W2057788907 countsByYear W20577889072014 @default.
- W2057788907 countsByYear W20577889072015 @default.
- W2057788907 countsByYear W20577889072016 @default.
- W2057788907 countsByYear W20577889072017 @default.
- W2057788907 countsByYear W20577889072018 @default.
- W2057788907 countsByYear W20577889072019 @default.
- W2057788907 countsByYear W20577889072020 @default.
- W2057788907 countsByYear W20577889072022 @default.
- W2057788907 crossrefType "journal-article" @default.
- W2057788907 hasAuthorship W2057788907A5006777495 @default.
- W2057788907 hasAuthorship W2057788907A5020825003 @default.
- W2057788907 hasAuthorship W2057788907A5032539966 @default.
- W2057788907 hasAuthorship W2057788907A5038567260 @default.
- W2057788907 hasAuthorship W2057788907A5045546704 @default.
- W2057788907 hasAuthorship W2057788907A5064384359 @default.
- W2057788907 hasAuthorship W2057788907A5085260473 @default.
- W2057788907 hasBestOaLocation W20577889071 @default.
- W2057788907 hasConcept C101762097 @default.
- W2057788907 hasConcept C104317684 @default.
- W2057788907 hasConcept C107459253 @default.
- W2057788907 hasConcept C123298856 @default.
- W2057788907 hasConcept C127561419 @default.
- W2057788907 hasConcept C134320426 @default.
- W2057788907 hasConcept C145103041 @default.
- W2057788907 hasConcept C148738053 @default.
- W2057788907 hasConcept C150194340 @default.