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- W2057800821 abstract "Abstract In shake-flask cultures, without renewal of the atmosphere, populations of L cells grew from 0.1 to about 0.8 million cells/ml in 4 days; ERK cell populations grew from 0.1 to about 0.5 million/ml in 4 days. The optimum tonicities for growth of L cells and ERK cells in shake-flask cultures were 363 and 289 m osm (ideal)/l respectively. Tonicity was more critical for the growth of ERK cells in suspension than in monolayer culture. Optimum concentrations of vitamins and serum were determined. Addition of bovine serum albumin (Fraction V) in the presence of serum had an irregular growth-promoting effect. Addition of a trace of MnCl 2 stimulated growth of ERK cells. Catalase (1 μg/ml) had a growth-promoting effect on ERK cells when the inoculum size was less than about 50,000 cells/ml." @default.
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- W2057800821 modified "2023-09-25" @default.
- W2057800821 title "Environmental influences on growth of L and ERK mammalian cells in shake-flask cultures" @default.
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- W2057800821 doi "https://doi.org/10.1016/0014-4827(64)90004-7" @default.
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