FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2057804993> ?p ?o ?g. }

• W2057804993 endingPage "3999" @default.
• W2057804993 startingPage "3995" @default.
• W2057804993 abstract "A general procedure is described for the production of human monoclonal antibodies from peripheral blood lymphocytes immunized in vitro against T-cell-dependent antigens. These lymphocytes immunized in culture were used to produce human-human or human-mouse hybridomas secreting monoclonal antibodies specific for digoxin, hemocyanin, a recombinant fragment of the gp120 envelope glycoprotein of human immunodeficiency virus (PB1), or a melanoma-associated antigen (p97). Depletion of a lysosome-rich cell population, containing large granular lymphocytes, monocytes, cytotoxic T cells, and a subset of CD8-positive T cells, was shown to be crucial before the cells could be immunized in vitro. This depletion was accomplished by treating the peripheral blood lymphocytes with the lysosomotropic agent L-leucine methyl ester. In addition, the in vitro immunization had to be supported by interleukin 2, gamma-interferon, and B-cell growth and differentiation factors, derived from irradiated, pokeweed-mitogen-stimulated human T cells. The production of human monoclonal antibodies from primary, antigen-specifically activated peripheral lymphocytes might obviate the need to immunize volunteers or patients." @default.
• W2057804993 created "2016-06-24" @default.
• W2057804993 creator A5031928636 @default.
• W2057804993 creator A5036600365 @default.
• W2057804993 creator A5086974017 @default.
• W2057804993 date "1988-06-01" @default.
• W2057804993 modified "2023-09-24" @default.
• W2057804993 title "Human monoclonal antibodies produced by primary in vitro immunization of peripheral blood lymphocytes." @default.
• W2057804993 cites W1218654354 @default.
• W2057804993 cites W1483824075 @default.
• W2057804993 cites W1489355697 @default.
• W2057804993 cites W1494816908 @default.
• W2057804993 cites W1499562567 @default.
• W2057804993 cites W1513889144 @default.
• W2057804993 cites W1564599513 @default.
• W2057804993 cites W157581341 @default.
• W2057804993 cites W1608418211 @default.
• W2057804993 cites W1697405926 @default.
• W2057804993 cites W1966108334 @default.
• W2057804993 cites W1987572262 @default.
• W2057804993 cites W1988007674 @default.
• W2057804993 cites W1989649611 @default.
• W2057804993 cites W1996815229 @default.
• W2057804993 cites W2002691231 @default.
• W2057804993 cites W2006311667 @default.
• W2057804993 cites W2011825464 @default.
• W2057804993 cites W2024563799 @default.
• W2057804993 cites W2027559384 @default.
• W2057804993 cites W2047708395 @default.
• W2057804993 cites W2059987855 @default.
• W2057804993 cites W2081549017 @default.
• W2057804993 cites W2094390593 @default.
• W2057804993 cites W2126064271 @default.
• W2057804993 cites W2133880058 @default.
• W2057804993 cites W2148404862 @default.
• W2057804993 cites W2152486077 @default.
• W2057804993 cites W2272276626 @default.
• W2057804993 cites W2433840757 @default.
• W2057804993 doi "https://doi.org/10.1073/pnas.85.11.3995" @default.
• W2057804993 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/280347" @default.
• W2057804993 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3131770" @default.
• W2057804993 hasPublicationYear "1988" @default.
• W2057804993 type Work @default.
• W2057804993 sameAs 2057804993 @default.
• W2057804993 citedByCount "103" @default.
• W2057804993 countsByYear W20578049932012 @default.
• W2057804993 countsByYear W20578049932013 @default.
• W2057804993 countsByYear W20578049932014 @default.
• W2057804993 countsByYear W20578049932016 @default.
• W2057804993 countsByYear W20578049932017 @default.
• W2057804993 countsByYear W20578049932018 @default.
• W2057804993 countsByYear W20578049932019 @default.
• W2057804993 countsByYear W20578049932020 @default.
• W2057804993 countsByYear W20578049932021 @default.
• W2057804993 countsByYear W20578049932023 @default.
• W2057804993 crossrefType "journal-article" @default.
• W2057804993 hasAuthorship W2057804993A5031928636 @default.
• W2057804993 hasAuthorship W2057804993A5036600365 @default.
• W2057804993 hasAuthorship W2057804993A5086974017 @default.
• W2057804993 hasBestOaLocation W20578049932 @default.
• W2057804993 hasConcept C137061746 @default.
• W2057804993 hasConcept C147483822 @default.
• W2057804993 hasConcept C153911025 @default.
• W2057804993 hasConcept C154317977 @default.
• W2057804993 hasConcept C159047783 @default.
• W2057804993 hasConcept C159654299 @default.
• W2057804993 hasConcept C202751555 @default.
• W2057804993 hasConcept C203014093 @default.
• W2057804993 hasConcept C2781005381 @default.
• W2057804993 hasConcept C2908647359 @default.
• W2057804993 hasConcept C2992881475 @default.
• W2057804993 hasConcept C542903549 @default.
• W2057804993 hasConcept C55493867 @default.
• W2057804993 hasConcept C71924100 @default.
• W2057804993 hasConcept C86803240 @default.
• W2057804993 hasConcept C99454951 @default.
• W2057804993 hasConceptScore W2057804993C137061746 @default.
• W2057804993 hasConceptScore W2057804993C147483822 @default.
• W2057804993 hasConceptScore W2057804993C153911025 @default.
• W2057804993 hasConceptScore W2057804993C154317977 @default.
• W2057804993 hasConceptScore W2057804993C159047783 @default.
• W2057804993 hasConceptScore W2057804993C159654299 @default.
• W2057804993 hasConceptScore W2057804993C202751555 @default.
• W2057804993 hasConceptScore W2057804993C203014093 @default.
• W2057804993 hasConceptScore W2057804993C2781005381 @default.
• W2057804993 hasConceptScore W2057804993C2908647359 @default.
• W2057804993 hasConceptScore W2057804993C2992881475 @default.
• W2057804993 hasConceptScore W2057804993C542903549 @default.
• W2057804993 hasConceptScore W2057804993C55493867 @default.
• W2057804993 hasConceptScore W2057804993C71924100 @default.
• W2057804993 hasConceptScore W2057804993C86803240 @default.
• W2057804993 hasConceptScore W2057804993C99454951 @default.
• W2057804993 hasIssue "11" @default.
• W2057804993 hasLocation W20578049931 @default.
• W2057804993 hasLocation W20578049932 @default.
• W2057804993 hasLocation W20578049933 @default.
• W2057804993 hasOpenAccess W2057804993 @default.
• W2057804993 hasPrimaryLocation W20578049931 @default.

• next