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- W2057808619 endingPage "1043" @default.
- W2057808619 startingPage "1035" @default.
- W2057808619 abstract "Xanthomonas campestris pv. campestris (Xcc), a close relative to Pseudomonas aeruginosa, is the pathogen causing black rot in cruciferous plants. In P. aeruginosa, FleQ serves as a cognate activator of sigma54 in transcription from several sigma54-dependent promoters of flagellar genes. These P. aeruginosa promoters have been analyzed for FleQ-binding sequences; however, no consensus was deduced. Xcc, although lacks fleSR, has a fleQ homologue residing among over 40 contiguously clustered flagellar genes. A fleQ mutant, Xc17fleQ, constructed by insertional mutation is deficient in FleQ protein, non-flagellated, and immobile. Transcriptional fusion assays on six putative sigma54-dependent promoters of the flagellar genes, fliE, fliQ, fliL, flgG, flgB, and flhF, indicated that each of them is also FleQ dependent. Each of these promoters has a sequence with weak consensus to 5'-gaaacCCgccgCcgctTt-3', immediately upstream of the predicted sigma54-binding site, with an imperfect inverted repeat containing a GC-rich center flanked by several A and T at 5'- and 3'-ends, respectively. Replacing this region in fliE promoter with a HindIII recognition sequence abolished the transcription, indicating that this region responds to transcription activation by FleQ." @default.
- W2057808619 created "2016-06-24" @default.
- W2057808619 creator A5046049133 @default.
- W2057808619 creator A5064144151 @default.
- W2057808619 creator A5070344536 @default.
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- W2057808619 date "2005-10-01" @default.
- W2057808619 modified "2023-09-23" @default.
- W2057808619 title "Deduction of upstream sequences of Xanthomonas campestris flagellar genes responding to transcription activation by FleQ" @default.
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- W2057808619 doi "https://doi.org/10.1016/j.bbrc.2005.07.171" @default.
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