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• W2057863067 abstract "Objective: The process of gametogenesis in the fruitfly, Drosophila melanogaster, is remarkably similar to that of mammals, and it is highly likely that analogous genes and molecular pathways regulate this conserved and fundamental biological process. Using Drosophila, a powerful genetic and research tool, molecular identity of one of the genes required during gametogenesis, zpg, was elucidated and its biological function was investigated by phenotypic analysis. Design: A forward genetic approach was taken to clone the genomic region of the zpg locus and identify the molecular nature of the gene protein product. Materials and Methods: Drosophila strains from chemical and insertional mutagenesis that are allelic to the zpg locus were phenotypically characterized in detail by immunofluorescence, in-situ hybridization, and enhancer trap lines that mark specific cell types in the very early steps of spermatogenesis. Genomic region of the zpg locus was cloned by plasmid rescue off the transposon tagged allele and screening of the genomic library. Candidate genes were identified by screening the cDNA library and sequence analysis. The precise identity of the gene was determined by rescuing the mutant phenotype with a transgene containing the candidate transcript. Results: The zpg gene appears to be required in the very early steps of male and female gametogenesis in Drosophila melanogaster. Flies mutant for the zpg gene are viable but sterile with tiny gonads due to failure during the mitotic amplification stage of spermatogonial and oogonial cells. Phenotypic analysis using immunofluorescence, in-situ hybridization, and enhancer trap lines indicate that both somatic and germ line cell types comprising the early steps of gametogenesis are present. The germ cells, however, undergo premature apoptosis instead of proliferation and subsequent differentiation. The molecular identity of the zpg gene is a novel protein highly homologous to gap junction proteins in invertebrates with four putative transmembrane domains and conserved cysteine residues in the extracellular portion. The gene is expressed specifically in germ cells during organogenesis in Drosophila. Conclusion: Mutation in zpg, a novel germ cell specific gap junction protein in Drosophila, produce male and female hypogonadism. The zpg gene may be analogous to the gonadal and tissue specific connexins in the mouse, previously shown to be required for gametogenesis and maintenance of meiotic arrest in pre-ovulatory oocytes. Further molecular and biochemical characterization of the gene product is underway to understand its mechanism of its action." @default.
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• W2057863067 date "2000-09-01" @default.
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• W2057863067 title "Role of a Novel Gap Junction Protein in Drosophila melanogaster, zero population growth (zpg), in Spermatogonial and Oogonial Cells" @default.
• W2057863067 doi "https://doi.org/10.1016/s0015-0282(00)01088-8" @default.
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