Matches in SemOpenAlex for { <https://semopenalex.org/work/W2057882511> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2057882511 endingPage "e57142" @default.
- W2057882511 startingPage "e57142" @default.
- W2057882511 abstract "The fundamental phenotypes of growth rate, size and morphology are the result of complex interactions between genotype and environment. We developed a high-throughput software application, WormSizer, which computes size and shape of nematodes from brightfield images. Existing methods for estimating volume either coarsely model the nematode as a cylinder or assume the worm shape or opacity is invariant. Our estimate is more robust to changes in morphology or optical density as it only assumes radial symmetry. This open source software is written as a plugin for the well-known image-processing framework Fiji/ImageJ. It may therefore be extended easily. We evaluated the technical performance of this framework, and we used it to analyze growth and shape of several canonical Caenorhabditis elegans mutants in a developmental time series. We confirm quantitatively that a Dumpy (Dpy) mutant is short and fat and that a Long (Lon) mutant is long and thin. We show that daf-2 insulin-like receptor mutants are larger than wild-type upon hatching but grow slow, and WormSizer can distinguish dauer larvae from normal larvae. We also show that a Small (Sma) mutant is actually smaller than wild-type at all stages of larval development. WormSizer works with Uncoordinated (Unc) and Roller (Rol) mutants as well, indicating that it can be used with mutants despite behavioral phenotypes. We used our complete data set to perform a power analysis, giving users a sense of how many images are needed to detect different effect sizes. Our analysis confirms and extends on existing phenotypic characterization of well-characterized mutants, demonstrating the utility and robustness of WormSizer." @default.
- W2057882511 created "2016-06-24" @default.
- W2057882511 creator A5027310847 @default.
- W2057882511 creator A5047717620 @default.
- W2057882511 creator A5079160666 @default.
- W2057882511 date "2013-02-22" @default.
- W2057882511 modified "2023-10-07" @default.
- W2057882511 title "WormSizer: High-throughput Analysis of Nematode Size and Shape" @default.
- W2057882511 cites W1571414277 @default.
- W2057882511 cites W1978818813 @default.
- W2057882511 cites W1989600725 @default.
- W2057882511 cites W2017568944 @default.
- W2057882511 cites W2037586865 @default.
- W2057882511 cites W2042567314 @default.
- W2057882511 cites W2054183111 @default.
- W2057882511 cites W2075851722 @default.
- W2057882511 cites W2108257056 @default.
- W2057882511 cites W2112510720 @default.
- W2057882511 cites W2132471426 @default.
- W2057882511 cites W2144498949 @default.
- W2057882511 cites W2167279371 @default.
- W2057882511 cites W2315391777 @default.
- W2057882511 cites W2500738484 @default.
- W2057882511 doi "https://doi.org/10.1371/journal.pone.0057142" @default.
- W2057882511 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3579787" @default.
- W2057882511 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23451165" @default.
- W2057882511 hasPublicationYear "2013" @default.
- W2057882511 type Work @default.
- W2057882511 sameAs 2057882511 @default.
- W2057882511 citedByCount "102" @default.
- W2057882511 countsByYear W20578825112014 @default.
- W2057882511 countsByYear W20578825112015 @default.
- W2057882511 countsByYear W20578825112016 @default.
- W2057882511 countsByYear W20578825112017 @default.
- W2057882511 countsByYear W20578825112018 @default.
- W2057882511 countsByYear W20578825112019 @default.
- W2057882511 countsByYear W20578825112020 @default.
- W2057882511 countsByYear W20578825112021 @default.
- W2057882511 countsByYear W20578825112022 @default.
- W2057882511 countsByYear W20578825112023 @default.
- W2057882511 crossrefType "journal-article" @default.
- W2057882511 hasAuthorship W2057882511A5027310847 @default.
- W2057882511 hasAuthorship W2057882511A5047717620 @default.
- W2057882511 hasAuthorship W2057882511A5079160666 @default.
- W2057882511 hasBestOaLocation W20578825111 @default.
- W2057882511 hasConcept C104317684 @default.
- W2057882511 hasConcept C127716648 @default.
- W2057882511 hasConcept C143065580 @default.
- W2057882511 hasConcept C183074962 @default.
- W2057882511 hasConcept C186060115 @default.
- W2057882511 hasConcept C18903297 @default.
- W2057882511 hasConcept C207583985 @default.
- W2057882511 hasConcept C2778830712 @default.
- W2057882511 hasConcept C2778944004 @default.
- W2057882511 hasConcept C54355233 @default.
- W2057882511 hasConcept C70721500 @default.
- W2057882511 hasConcept C86803240 @default.
- W2057882511 hasConceptScore W2057882511C104317684 @default.
- W2057882511 hasConceptScore W2057882511C127716648 @default.
- W2057882511 hasConceptScore W2057882511C143065580 @default.
- W2057882511 hasConceptScore W2057882511C183074962 @default.
- W2057882511 hasConceptScore W2057882511C186060115 @default.
- W2057882511 hasConceptScore W2057882511C18903297 @default.
- W2057882511 hasConceptScore W2057882511C207583985 @default.
- W2057882511 hasConceptScore W2057882511C2778830712 @default.
- W2057882511 hasConceptScore W2057882511C2778944004 @default.
- W2057882511 hasConceptScore W2057882511C54355233 @default.
- W2057882511 hasConceptScore W2057882511C70721500 @default.
- W2057882511 hasConceptScore W2057882511C86803240 @default.
- W2057882511 hasIssue "2" @default.
- W2057882511 hasLocation W20578825111 @default.
- W2057882511 hasLocation W20578825112 @default.
- W2057882511 hasLocation W20578825113 @default.
- W2057882511 hasLocation W20578825114 @default.
- W2057882511 hasLocation W20578825115 @default.
- W2057882511 hasLocation W20578825116 @default.
- W2057882511 hasOpenAccess W2057882511 @default.
- W2057882511 hasPrimaryLocation W20578825111 @default.
- W2057882511 hasRelatedWork W1577397597 @default.
- W2057882511 hasRelatedWork W2183871674 @default.
- W2057882511 hasRelatedWork W2356164561 @default.
- W2057882511 hasRelatedWork W2993659554 @default.
- W2057882511 hasRelatedWork W3131791460 @default.
- W2057882511 hasRelatedWork W390558816 @default.
- W2057882511 hasRelatedWork W4313474347 @default.
- W2057882511 hasRelatedWork W4384347930 @default.
- W2057882511 hasRelatedWork W4385516583 @default.
- W2057882511 hasRelatedWork W978202925 @default.
- W2057882511 hasVolume "8" @default.
- W2057882511 isParatext "false" @default.
- W2057882511 isRetracted "false" @default.
- W2057882511 magId "2057882511" @default.
- W2057882511 workType "article" @default.