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- W2057888971 abstract "Potential difference (p.d.), short-circuit current (SCC) and electrical resistance (Rt) across the isolated toad ciliary epithelium were measured under normal and various experimental conditions including altered ionic composition of the incubation medium, anoxia, the presence of 2,4-dinitrophenol (DNP), ouabain, thiocyanate (SCN−) or theophylline. Under normal conditions, the average values of p.d., SCC and Rt were 3·7 mV (epithelial side negative), 51 μA/cm2, and 88·5 Ωcm2, respectively (n = 29). In ion substitution experiments, SCC increased in proportion to the increase both in Na+ and Cl− concentration and showed a saturable tendency at higher concentration ranges. The elimination of either Na+ or Cl− from both sides abolished p.d. and SCC almost completely. An increase in K+ had a stimulatory effect on SCC at lower concentrations, while it showed an inhibitory effect at higher concentrations and K+ (Na+-free)-Ringer reversed the polarity of p.d. Anoxia (bubbling with N2), 1 mm-DNP and 1 mm-ouabain decreased p.d. and SCC when N2 or one of the drugs was applied to either side but the effects were more potent when the drug was applied to the epithelial side. On the other hand 10 mm-SCN− and 10 mm-theophylline caused a significant and reversible decrease in SCC only when added to the epithelial side. It is concluded that electrogenic Cl− transport which absolutely requires both Na+ and K+ is indispensable in the transepithelial ion transport in the toad ciliary body." @default.
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- W2057888971 date "1978-08-01" @default.
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- W2057888971 title "Characteristics of ion transport across the isolated ciliary epithelium of the toad as studied by electrical measurements" @default.
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- W2057888971 doi "https://doi.org/10.1016/0014-4835(78)90090-8" @default.
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