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- W2057970875 abstract "Unscheduled DNA synthesis (UDS) was demonstrated both in fresh slices of rabbit articular cartilage and in resting chondrocytes dissociated from it. Uptake of [3H]thymidine by chondrocytes treated with 10 mM hydroxyurea and irradiated with 254 nm ultraviolet light (20 J/m2) was measured by autoradiography. The UDS in the resting chondrocytes was minuscule compared with that of chondrocytes growing in monolayer culture. It was greater in resting cells from 3- than in 18-month old rabbits but persisted in animals up to 52 months of age. In both resting and cultured chondrocytes, UDS was greatly inhibited by aphidicolin (5 μg/ml). The finding of age-related decrease in UDS of resting chondrocytes, contrasted with previously demonstrated lack of it in dividing ones, raises questions about in vitro artefacts in studies of cellular senescence." @default.
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- W2057970875 date "1985-10-01" @default.
- W2057970875 modified "2023-10-18" @default.
- W2057970875 title "DNA repair by articular chondrocytes. III. Unscheduled DNA synthesis following ultraviolet light irradiation of resting cartilage" @default.
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- W2057970875 doi "https://doi.org/10.1016/0047-6374(85)90034-x" @default.
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