Matches in SemOpenAlex for { <https://semopenalex.org/work/W2057984012> ?p ?o ?g. }
- W2057984012 endingPage "25534" @default.
- W2057984012 startingPage "25522" @default.
- W2057984012 abstract "Protein glycosylation is a common post-translational modification, the effect of which on protein conformational and stability is incompletely understood. Here we have investigated the effects of glycosylation on the thermostability of Bacillus subtilis xylanase A (XynA) expressed in Pichia pastoris. Intact mass analysis of the heterologous wild-type XynA revealed two, three, or four Hex(8-16)GlcNAc2 modifications involving asparagine residues at positions 20, 25, 141, and 181. Molecular dynamics (MD) simulations of the XynA modified with various combinations of branched Hex9GlcNAc2 at these positions indicated a significant contribution from protein-glycan interactions to the overall energy of the glycoproteins. The effect of glycan content and glycosylation position on protein stability was evaluated by combinatorial mutagenesis of all six potential N-glycosylation sites. The majority of glycosylated enzymes expressed in P. pastoris presented increased thermostability in comparison with their unglycosylated counterparts expressed in Escherichia coli. Steric effects of multiple glycosylation events were apparent, and glycosylation position rather than the number of glycosylation events determined increases in thermostability. The MD simulations also indicated that clustered glycan chains tended to favor less stabilizing glycan-glycan interactions, whereas more dispersed glycosylation patterns favored stabilizing protein-glycan interactions." @default.
- W2057984012 created "2016-06-24" @default.
- W2057984012 creator A5009187210 @default.
- W2057984012 creator A5028069486 @default.
- W2057984012 creator A5038637869 @default.
- W2057984012 creator A5051970675 @default.
- W2057984012 creator A5058795992 @default.
- W2057984012 creator A5069059746 @default.
- W2057984012 date "2013-08-01" @default.
- W2057984012 modified "2023-10-01" @default.
- W2057984012 title "Engineering the Pattern of Protein Glycosylation Modulates the Thermostability of a GH11 Xylanase" @default.
- W2057984012 cites W185029269 @default.
- W2057984012 cites W1965710128 @default.
- W2057984012 cites W1966078827 @default.
- W2057984012 cites W1975699819 @default.
- W2057984012 cites W1992637173 @default.
- W2057984012 cites W2003099086 @default.
- W2057984012 cites W2004125187 @default.
- W2057984012 cites W2004816473 @default.
- W2057984012 cites W2009674410 @default.
- W2057984012 cites W2011732398 @default.
- W2057984012 cites W2015064119 @default.
- W2057984012 cites W2024191848 @default.
- W2057984012 cites W2026357888 @default.
- W2057984012 cites W2027560826 @default.
- W2057984012 cites W2028062152 @default.
- W2057984012 cites W2030303517 @default.
- W2057984012 cites W2034365731 @default.
- W2057984012 cites W2035784347 @default.
- W2057984012 cites W2039608986 @default.
- W2057984012 cites W2040470885 @default.
- W2057984012 cites W2045316115 @default.
- W2057984012 cites W2046714822 @default.
- W2057984012 cites W2047048276 @default.
- W2057984012 cites W2051403769 @default.
- W2057984012 cites W2053322857 @default.
- W2057984012 cites W2067093784 @default.
- W2057984012 cites W2067174909 @default.
- W2057984012 cites W2072086606 @default.
- W2057984012 cites W2073026684 @default.
- W2057984012 cites W2073808757 @default.
- W2057984012 cites W2100837269 @default.
- W2057984012 cites W2103945336 @default.
- W2057984012 cites W2107572441 @default.
- W2057984012 cites W2108179651 @default.
- W2057984012 cites W2110068022 @default.
- W2057984012 cites W2115499476 @default.
- W2057984012 cites W2117458899 @default.
- W2057984012 cites W2137741805 @default.
- W2057984012 cites W2149369491 @default.
- W2057984012 cites W2150402483 @default.
- W2057984012 cites W2159805086 @default.
- W2057984012 cites W2161605585 @default.
- W2057984012 cites W2170506326 @default.
- W2057984012 doi "https://doi.org/10.1074/jbc.m113.485953" @default.
- W2057984012 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3757213" @default.
- W2057984012 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23846692" @default.
- W2057984012 hasPublicationYear "2013" @default.
- W2057984012 type Work @default.
- W2057984012 sameAs 2057984012 @default.
- W2057984012 citedByCount "58" @default.
- W2057984012 countsByYear W20579840122014 @default.
- W2057984012 countsByYear W20579840122015 @default.
- W2057984012 countsByYear W20579840122016 @default.
- W2057984012 countsByYear W20579840122017 @default.
- W2057984012 countsByYear W20579840122018 @default.
- W2057984012 countsByYear W20579840122019 @default.
- W2057984012 countsByYear W20579840122020 @default.
- W2057984012 countsByYear W20579840122021 @default.
- W2057984012 countsByYear W20579840122022 @default.
- W2057984012 countsByYear W20579840122023 @default.
- W2057984012 crossrefType "journal-article" @default.
- W2057984012 hasAuthorship W2057984012A5009187210 @default.
- W2057984012 hasAuthorship W2057984012A5028069486 @default.
- W2057984012 hasAuthorship W2057984012A5038637869 @default.
- W2057984012 hasAuthorship W2057984012A5051970675 @default.
- W2057984012 hasAuthorship W2057984012A5058795992 @default.
- W2057984012 hasAuthorship W2057984012A5069059746 @default.
- W2057984012 hasBestOaLocation W20579840121 @default.
- W2057984012 hasConcept C104317684 @default.
- W2057984012 hasConcept C108625454 @default.
- W2057984012 hasConcept C147816474 @default.
- W2057984012 hasConcept C181199279 @default.
- W2057984012 hasConcept C185592680 @default.
- W2057984012 hasConcept C206212055 @default.
- W2057984012 hasConcept C2775895851 @default.
- W2057984012 hasConcept C2777313579 @default.
- W2057984012 hasConcept C2781216036 @default.
- W2057984012 hasConcept C2781372317 @default.
- W2057984012 hasConcept C30324644 @default.
- W2057984012 hasConcept C40767141 @default.
- W2057984012 hasConcept C55493867 @default.
- W2057984012 hasConceptScore W2057984012C104317684 @default.
- W2057984012 hasConceptScore W2057984012C108625454 @default.
- W2057984012 hasConceptScore W2057984012C147816474 @default.
- W2057984012 hasConceptScore W2057984012C181199279 @default.
- W2057984012 hasConceptScore W2057984012C185592680 @default.
- W2057984012 hasConceptScore W2057984012C206212055 @default.