Matches in SemOpenAlex for { <https://semopenalex.org/work/W2057992913> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2057992913 endingPage "722" @default.
- W2057992913 startingPage "715" @default.
- W2057992913 abstract "A rapid and convenient method of protein purification involves creating a fusion protein with glutathione S-transferase (GST) (Smith and Johnson, Gene 67, 31–40, 1988). In this report, we describe two vectors for the conditional expression of GST fusions in Saccharomyces cerevisiae. The parent plasmid is based on a high-copy, galactose-inducible shuttle vector previously described (Baldari et al., EMBO J. 6, 229–243, 1987). We have demonstrated the use of this system by creating fusions between GST and the yeast RAS2 gene. GST-Ras2 fusion proteins undergo the post-translational modifications required for Ras2p to become membrane localized. These vectors provide a useful system for the expression an dpurification of eukaryotic proteins requiring post-translational modification." @default.
- W2057992913 created "2016-06-24" @default.
- W2057992913 creator A5038489941 @default.
- W2057992913 creator A5040229377 @default.
- W2057992913 creator A5056551030 @default.
- W2057992913 date "1993-07-01" @default.
- W2057992913 modified "2023-10-17" @default.
- W2057992913 title "Vectors for the inducible overexpression of glutathione S-transferase fusion proteins in yeast" @default.
- W2057992913 cites W1556811458 @default.
- W2057992913 cites W1606923005 @default.
- W2057992913 cites W1945627345 @default.
- W2057992913 cites W1973782344 @default.
- W2057992913 cites W1995267722 @default.
- W2057992913 cites W1996223704 @default.
- W2057992913 cites W2008014792 @default.
- W2057992913 cites W2067045675 @default.
- W2057992913 cites W2068446924 @default.
- W2057992913 cites W2071523919 @default.
- W2057992913 cites W2100837269 @default.
- W2057992913 cites W2128093756 @default.
- W2057992913 cites W2178557511 @default.
- W2057992913 cites W4293247451 @default.
- W2057992913 cites W971382953 @default.
- W2057992913 doi "https://doi.org/10.1002/yea.320090705" @default.
- W2057992913 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8368005" @default.
- W2057992913 hasPublicationYear "1993" @default.
- W2057992913 type Work @default.
- W2057992913 sameAs 2057992913 @default.
- W2057992913 citedByCount "277" @default.
- W2057992913 countsByYear W20579929132012 @default.
- W2057992913 countsByYear W20579929132013 @default.
- W2057992913 countsByYear W20579929132014 @default.
- W2057992913 countsByYear W20579929132015 @default.
- W2057992913 countsByYear W20579929132016 @default.
- W2057992913 countsByYear W20579929132017 @default.
- W2057992913 countsByYear W20579929132018 @default.
- W2057992913 countsByYear W20579929132019 @default.
- W2057992913 countsByYear W20579929132020 @default.
- W2057992913 countsByYear W20579929132021 @default.
- W2057992913 countsByYear W20579929132022 @default.
- W2057992913 crossrefType "journal-article" @default.
- W2057992913 hasAuthorship W2057992913A5038489941 @default.
- W2057992913 hasAuthorship W2057992913A5040229377 @default.
- W2057992913 hasAuthorship W2057992913A5056551030 @default.
- W2057992913 hasConcept C104317684 @default.
- W2057992913 hasConcept C111829193 @default.
- W2057992913 hasConcept C123894998 @default.
- W2057992913 hasConcept C145640855 @default.
- W2057992913 hasConcept C153911025 @default.
- W2057992913 hasConcept C181199279 @default.
- W2057992913 hasConcept C22744801 @default.
- W2057992913 hasConcept C2776376580 @default.
- W2057992913 hasConcept C2776907368 @default.
- W2057992913 hasConcept C2777576037 @default.
- W2057992913 hasConcept C2779222958 @default.
- W2057992913 hasConcept C33161422 @default.
- W2057992913 hasConcept C40767141 @default.
- W2057992913 hasConcept C538909803 @default.
- W2057992913 hasConcept C55493867 @default.
- W2057992913 hasConcept C86803240 @default.
- W2057992913 hasConcept C92087593 @default.
- W2057992913 hasConceptScore W2057992913C104317684 @default.
- W2057992913 hasConceptScore W2057992913C111829193 @default.
- W2057992913 hasConceptScore W2057992913C123894998 @default.
- W2057992913 hasConceptScore W2057992913C145640855 @default.
- W2057992913 hasConceptScore W2057992913C153911025 @default.
- W2057992913 hasConceptScore W2057992913C181199279 @default.
- W2057992913 hasConceptScore W2057992913C22744801 @default.
- W2057992913 hasConceptScore W2057992913C2776376580 @default.
- W2057992913 hasConceptScore W2057992913C2776907368 @default.
- W2057992913 hasConceptScore W2057992913C2777576037 @default.
- W2057992913 hasConceptScore W2057992913C2779222958 @default.
- W2057992913 hasConceptScore W2057992913C33161422 @default.
- W2057992913 hasConceptScore W2057992913C40767141 @default.
- W2057992913 hasConceptScore W2057992913C538909803 @default.
- W2057992913 hasConceptScore W2057992913C55493867 @default.
- W2057992913 hasConceptScore W2057992913C86803240 @default.
- W2057992913 hasConceptScore W2057992913C92087593 @default.
- W2057992913 hasIssue "7" @default.
- W2057992913 hasLocation W20579929131 @default.
- W2057992913 hasLocation W20579929132 @default.
- W2057992913 hasOpenAccess W2057992913 @default.
- W2057992913 hasPrimaryLocation W20579929131 @default.
- W2057992913 hasRelatedWork W2057992913 @default.
- W2057992913 hasRelatedWork W2353726216 @default.
- W2057992913 hasRelatedWork W2355848544 @default.
- W2057992913 hasRelatedWork W2359842457 @default.
- W2057992913 hasRelatedWork W2359862199 @default.
- W2057992913 hasRelatedWork W2373882081 @default.
- W2057992913 hasRelatedWork W2377820123 @default.
- W2057992913 hasRelatedWork W2382615743 @default.
- W2057992913 hasRelatedWork W2384592831 @default.
- W2057992913 hasRelatedWork W2389269354 @default.
- W2057992913 hasVolume "9" @default.
- W2057992913 isParatext "false" @default.
- W2057992913 isRetracted "false" @default.
- W2057992913 magId "2057992913" @default.
- W2057992913 workType "article" @default.