FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058019210> ?p ?o ?g. }

• W2058019210 abstract "Many pathologies are characterized by poor blood vessel growth and reduced nutrient delivery to the surrounding tissue, introducing a need for tissue engineered blood vessels. Our lab has developed a 3D co-culture method to grow interconnected networks of pericyte-invested capillaries, which can anastamose with host vasculature following implantation to restore blood flow to ischemic tissues. However, if the engineered vessels contain endothelial cells (ECs) that are misaligned or contain wide junctional gaps, they may function improperly and behave more like the pathologic vessels that nourish tumors. The purpose of this study was to test the resistance to permeability of these networks in vitro, grown with different stromal cell types, as a metric of vessel functionality. A fluorescent dextran tracer was used to visualize transport across the endothelium and the pixel intensity was quantified using a customized MATLAB algorithm. In fibroblast-EC co-cultures, the dextran tracer easily penetrated through the vessel wall and permeability was high through the first 5 days of culture, indicative of vessel immaturity. Beyond day 5, dextran accumulated at the periphery of the vessel, with very little transported across the endothelium. Quantitatively, permeability dropped from initial levels of 61% to 39% after 7 days, and to 7% after 2 weeks. When ECs were co-cultured with bone marrow-derived mesenchymal stem cells (MSCs) or adipose-derived stem cells (AdSCs), much tighter control of permeability was achieved. Relative to the EC-fibroblast co-cultures, permeabilities were reduced 41% for the EC-MSC co-cultures and 50% for the EC-AdSC co-cultures after 3 days of culture. By day 14, these permeabilities decreased by 68% and 77% over the EC-fibroblast cultures. Co-cultures containing stem cells exhibit elevated VE-cadherin levels and more prominent EC-EC junctional complexes when compared to cultures containing fibroblasts. These data suggest the stromal cell identity influences the functionality and physiologic relevance of engineered capillary networks." @default.
• W2058019210 created "2016-06-24" @default.
• W2058019210 creator A5027057361 @default.
• W2058019210 creator A5090910003 @default.
• W2058019210 date "2011-01-01" @default.
• W2058019210 modified "2023-10-14" @default.
• W2058019210 title "Assessing the permeability of engineered capillary networks in a 3D culture." @default.
• W2058019210 cites W1495287184 @default.
• W2058019210 cites W1519008817 @default.
• W2058019210 cites W1975292043 @default.
• W2058019210 cites W1983444416 @default.
• W2058019210 cites W1984780868 @default.
• W2058019210 cites W1987535525 @default.
• W2058019210 cites W1990793260 @default.
• W2058019210 cites W1993866602 @default.
• W2058019210 cites W2021689192 @default.
• W2058019210 cites W2025036947 @default.
• W2058019210 cites W2030426885 @default.
• W2058019210 cites W2041635943 @default.
• W2058019210 cites W2047126924 @default.
• W2058019210 cites W2047470479 @default.
• W2058019210 cites W2051534823 @default.
• W2058019210 cites W2061358820 @default.
• W2058019210 cites W2064461365 @default.
• W2058019210 cites W2071801522 @default.
• W2058019210 cites W2089004231 @default.
• W2058019210 cites W2098799479 @default.
• W2058019210 cites W2108813969 @default.
• W2058019210 cites W2115368694 @default.
• W2058019210 cites W2122612027 @default.
• W2058019210 cites W2123793642 @default.
• W2058019210 cites W2126810504 @default.
• W2058019210 cites W2129477690 @default.
• W2058019210 cites W2138811791 @default.
• W2058019210 cites W2141126811 @default.
• W2058019210 cites W2152442991 @default.
• W2058019210 cites W2187990808 @default.
• W2058019210 cites W2198940906 @default.
• W2058019210 cites W4211147424 @default.
• W2058019210 doi "https://doi.org/10.1371/journal.pone.0022086" @default.
• W2058019210 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3131402" @default.
• W2058019210 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21760956" @default.
• W2058019210 hasPublicationYear "2011" @default.
• W2058019210 type Work @default.
• W2058019210 sameAs 2058019210 @default.
• W2058019210 citedByCount "58" @default.
• W2058019210 countsByYear W20580192102012 @default.
• W2058019210 countsByYear W20580192102013 @default.
• W2058019210 countsByYear W20580192102014 @default.
• W2058019210 countsByYear W20580192102015 @default.
• W2058019210 countsByYear W20580192102016 @default.
• W2058019210 countsByYear W20580192102017 @default.
• W2058019210 countsByYear W20580192102018 @default.
• W2058019210 countsByYear W20580192102019 @default.
• W2058019210 countsByYear W20580192102020 @default.
• W2058019210 countsByYear W20580192102021 @default.
• W2058019210 countsByYear W20580192102022 @default.
• W2058019210 countsByYear W20580192102023 @default.
• W2058019210 crossrefType "journal-article" @default.
• W2058019210 hasAuthorship W2058019210A5027057361 @default.
• W2058019210 hasAuthorship W2058019210A5090910003 @default.
• W2058019210 hasBestOaLocation W20580192101 @default.
• W2058019210 hasConcept C113045295 @default.
• W2058019210 hasConcept C120882062 @default.
• W2058019210 hasConcept C123012128 @default.
• W2058019210 hasConcept C134018914 @default.
• W2058019210 hasConcept C136229726 @default.
• W2058019210 hasConcept C142724271 @default.
• W2058019210 hasConcept C16930146 @default.
• W2058019210 hasConcept C171089720 @default.
• W2058019210 hasConcept C185592680 @default.
• W2058019210 hasConcept C198826908 @default.
• W2058019210 hasConcept C202751555 @default.
• W2058019210 hasConcept C203014093 @default.
• W2058019210 hasConcept C2776992346 @default.
• W2058019210 hasConcept C2777339698 @default.
• W2058019210 hasConcept C2777663904 @default.
• W2058019210 hasConcept C2780007613 @default.
• W2058019210 hasConcept C2780381497 @default.
• W2058019210 hasConcept C28328180 @default.
• W2058019210 hasConcept C41625074 @default.
• W2058019210 hasConcept C49892992 @default.
• W2058019210 hasConcept C54355233 @default.
• W2058019210 hasConcept C55493867 @default.
• W2058019210 hasConcept C71924100 @default.
• W2058019210 hasConcept C81885089 @default.
• W2058019210 hasConcept C86803240 @default.
• W2058019210 hasConcept C95444343 @default.
• W2058019210 hasConceptScore W2058019210C113045295 @default.
• W2058019210 hasConceptScore W2058019210C120882062 @default.
• W2058019210 hasConceptScore W2058019210C123012128 @default.
• W2058019210 hasConceptScore W2058019210C134018914 @default.
• W2058019210 hasConceptScore W2058019210C136229726 @default.
• W2058019210 hasConceptScore W2058019210C142724271 @default.
• W2058019210 hasConceptScore W2058019210C16930146 @default.
• W2058019210 hasConceptScore W2058019210C171089720 @default.
• W2058019210 hasConceptScore W2058019210C185592680 @default.
• W2058019210 hasConceptScore W2058019210C198826908 @default.
• W2058019210 hasConceptScore W2058019210C202751555 @default.
• W2058019210 hasConceptScore W2058019210C203014093 @default.

• next