FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058045221> ?p ?o ?g. }

• W2058045221 endingPage "318" @default.
• W2058045221 startingPage "289" @default.
• W2058045221 abstract "1. The activation by Na ions and the effect of the anti-L antibody on the sodium pump of low-potassium type (LK) erythrocytes, have been studied by measuring ouabain-sensitive ATPase activity of red cell membranes of LK goats. The experimental data were first corrected for incomplete occupation of the external K sites of the pump, using a saturation function obtained from influx experiments.2. Double-reciprocal plots of the corrected rates against Na concentration at various fixed K concentrations, yield a pattern of competitive K inhibition when it is assumed that three equivalent sodium sites take part in the internal activation of LK-(Na+K)-ATPase. The dissociation constant of Na at each site (K(m)) lies between 10 and 20 mM and that of K as competitive inhibitor (K(i)), between 1.5 and 4.5 mM.3. The maximal rate of hydrolysis of LK goat (Na + K)-ATPase is not different from those usually obtained with the high-potassium type (HK) red cell enzyme. Then, the low pumping rate of LK erythrocytes in physiological conditions is only reflecting the poor Na affinity, both absolute and relative, at the internal Na sites of their sodium pumps.4. The stimulation of the ouabain-sensitive ATPase activity by sensitization of the membranes with anti-L serum, is mediated by a threefold reduction of the K(m)/K(i) ratio at each site. K(m) decreases by a factor of 10, but there is also a smaller diminution of K(i). The maximal rate of hydrolysis, however, is unchanged by the anti-L treatment. The least-squares fitting of the pooled data by the rate equation, converges better with less than three and more than two equivalent sodium sites.5. The affinity sequence at two external K sites of the LK goat erythrocyte sodium pump, determined in the presence of 100 mM external Na, is Rb > K > Cs. It is obtained from the concentration dependence in influx experiments, and is the same as reported for human red cells.6. Cubic-root Dixon plots of the corrected ouabain-sensitive ATPase activity against the concentration of K and its congeners, show the sequence Tl > K > Rb > Na > Cs for the affinities at the internal cation sites of the LK sodium pump. Anti-L treatment decreases the relative magnitude of Na and Cs selectivities, it being not certain whether a Rb-Na transition then occurs.7. The results are discussed in terms of possible mechanisms whereby the sodium pump of LK and HK red cells may adjust the properties of their cation sites upon translocation of monovalent cations." @default.
• W2058045221 created "2016-06-24" @default.
• W2058045221 creator A5002228204 @default.
• W2058045221 creator A5064103246 @default.
• W2058045221 date "1977-09-01" @default.
• W2058045221 modified "2023-09-25" @default.
• W2058045221 title "The interaction of monovalent cations with the sodium pump of low-potassium goat erythrocytes" @default.
• W2058045221 cites W1246567457 @default.
• W2058045221 cites W1431418 @default.
• W2058045221 cites W1480115832 @default.
• W2058045221 cites W1500442791 @default.
• W2058045221 cites W1542250560 @default.
• W2058045221 cites W1548269463 @default.
• W2058045221 cites W1551234630 @default.
• W2058045221 cites W1587174601 @default.
• W2058045221 cites W1608725638 @default.
• W2058045221 cites W1969088712 @default.
• W2058045221 cites W1969183302 @default.
• W2058045221 cites W1979826967 @default.
• W2058045221 cites W1979845718 @default.
• W2058045221 cites W1985694132 @default.
• W2058045221 cites W1986552268 @default.
• W2058045221 cites W1992214936 @default.
• W2058045221 cites W2004825492 @default.
• W2058045221 cites W2006238353 @default.
• W2058045221 cites W2007656190 @default.
• W2058045221 cites W2026283032 @default.
• W2058045221 cites W2048582710 @default.
• W2058045221 cites W2054418233 @default.
• W2058045221 cites W2062955242 @default.
• W2058045221 cites W2080247800 @default.
• W2058045221 cites W2084530235 @default.
• W2058045221 cites W2086291161 @default.
• W2058045221 cites W2098298014 @default.
• W2058045221 cites W2101258329 @default.
• W2058045221 cites W2121490515 @default.
• W2058045221 cites W2126996448 @default.
• W2058045221 cites W2133877107 @default.
• W2058045221 cites W2134089657 @default.
• W2058045221 cites W2144089964 @default.
• W2058045221 cites W2144382479 @default.
• W2058045221 cites W2147542552 @default.
• W2058045221 cites W2162293702 @default.
• W2058045221 cites W2209850193 @default.
• W2058045221 cites W2397861637 @default.
• W2058045221 cites W2398443655 @default.
• W2058045221 cites W2417695509 @default.
• W2058045221 cites W2423507188 @default.
• W2058045221 cites W2434875071 @default.
• W2058045221 cites W92654806 @default.
• W2058045221 doi "https://doi.org/10.1113/jphysiol.1977.sp012001" @default.
• W2058045221 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1353618" @default.
• W2058045221 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/144181" @default.
• W2058045221 hasPublicationYear "1977" @default.
• W2058045221 type Work @default.
• W2058045221 sameAs 2058045221 @default.
• W2058045221 citedByCount "14" @default.
• W2058045221 countsByYear W20580452212013 @default.
• W2058045221 crossrefType "journal-article" @default.
• W2058045221 hasAuthorship W2058045221A5002228204 @default.
• W2058045221 hasAuthorship W2058045221A5064103246 @default.
• W2058045221 hasBestOaLocation W20580452212 @default.
• W2058045221 hasConcept C102931765 @default.
• W2058045221 hasConcept C121332964 @default.
• W2058045221 hasConcept C12554922 @default.
• W2058045221 hasConcept C141315368 @default.
• W2058045221 hasConcept C147789679 @default.
• W2058045221 hasConcept C148898269 @default.
• W2058045221 hasConcept C178790620 @default.
• W2058045221 hasConcept C181199279 @default.
• W2058045221 hasConcept C185592680 @default.
• W2058045221 hasConcept C23265538 @default.
• W2058045221 hasConcept C2779133857 @default.
• W2058045221 hasConcept C41625074 @default.
• W2058045221 hasConcept C517785266 @default.
• W2058045221 hasConcept C537181965 @default.
• W2058045221 hasConcept C55493867 @default.
• W2058045221 hasConcept C62520636 @default.
• W2058045221 hasConcept C86803240 @default.
• W2058045221 hasConcept C93391505 @default.
• W2058045221 hasConcept C94412978 @default.
• W2058045221 hasConceptScore W2058045221C102931765 @default.
• W2058045221 hasConceptScore W2058045221C121332964 @default.
• W2058045221 hasConceptScore W2058045221C12554922 @default.
• W2058045221 hasConceptScore W2058045221C141315368 @default.
• W2058045221 hasConceptScore W2058045221C147789679 @default.
• W2058045221 hasConceptScore W2058045221C148898269 @default.
• W2058045221 hasConceptScore W2058045221C178790620 @default.
• W2058045221 hasConceptScore W2058045221C181199279 @default.
• W2058045221 hasConceptScore W2058045221C185592680 @default.
• W2058045221 hasConceptScore W2058045221C23265538 @default.
• W2058045221 hasConceptScore W2058045221C2779133857 @default.
• W2058045221 hasConceptScore W2058045221C41625074 @default.
• W2058045221 hasConceptScore W2058045221C517785266 @default.
• W2058045221 hasConceptScore W2058045221C537181965 @default.
• W2058045221 hasConceptScore W2058045221C55493867 @default.
• W2058045221 hasConceptScore W2058045221C62520636 @default.
• W2058045221 hasConceptScore W2058045221C86803240 @default.

• next