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- W2058045835 abstract "In human osteosarcoma MG63 cells, the effect of desipramine, an antidepressant, on intracellular Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]<sub>i</sub>) was measured by using fura-2. Desipramine (>10 µmol/l) caused a rapid and sustained rise of [Ca<sup>2+</sup>]<sub>i</sub> in a concentration-dependent manner (EC<sub>50</sub> = 200 µmol/l). Desipramine-induced [Ca<sup>2+</sup>]<sub>i</sub> rise was prevented by 80% by removal of extracellular Ca<sup>2+</sup> but was not altered by voltage-gated Ca<sup>2+</sup> channel blockers. In Ca<sup>2+</sup>-free medium, thapsigargin, an inhibitor of the endoplasmic reticulum (ER) Ca<sup>2+</sup>-ATPase, caused a monophasic [Ca<sup>2+</sup>]<sub>i</sub> rise, after which the increasing effect of desipramine on [Ca<sup>2+</sup>]<sub>i</sub> was abolished; also, pretreatment with desipramine partly reduced thapsigargin-induced [Ca<sup>2+</sup>]<sub>i</sub> increase. U73122, an inhibitor of phospholipase C, did not affect desipramine-induced [Ca<sup>2+</sup>]<sub>i</sub> rise. Overnight incubation with 10 µmol/l desipramine did not alter cell proliferation, but killed 32 and 89% of cells at concentrations of 100 and 200 µmol/l, respectively. These findings suggest that desipramine rapidly increases [Ca<sup>2+</sup>]<sub>i</sub> in osteoblasts by stimulating both extracellular Ca<sup>2+</sup> influx and intracellular Ca<sup>2+</sup> release, and is cytotoxic at high concentrations." @default.
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- W2058045835 date "2003-01-01" @default.
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- W2058045835 title "Effect of the Antidepressant Desipramine on Cytosolic Ca<sup>2+</sup> Movement and Proliferation in Human Osteosarcoma Cells" @default.
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- W2058045835 doi "https://doi.org/10.1159/000073663" @default.
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