Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058049327> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2058049327 abstract "Procathepsin H in kidney and liver microsomal lumen was identified to have a molecular mass of 41 kDa by immunoblot analysis. The proenzyme was then concentrated by applying the microsomal contents to a concanavalin A-Sepharose column. When the concanavalin A-adsorbed fraction was incubated at pH 4.0 at 20 degrees C, the activity measured with synthetic substrate increased 3.5 times over that of the control after 24 h incubation. Immunoblot analysis showed that acidic treatment caused the disappearance of procathepsin H. Thus the proenzyme might be processed to the mature enzyme under acidic conditions. The marked increase of enzymatic activity and the conversion of proenzyme were completely blocked with pepstatin which is a potent inhibitor of aspartic proteases. These results suggested that a protease for processing procathepsin H might be cathepsin D, a major lysosomal aspartic protease. Therefore, procathepsin H seems to be synthesized first in the enzymatically inactive form in endoplasmic reticulum and successively converted into the active form in lysosomes during biosynthesis." @default.
- W2058049327 created "2016-06-24" @default.
- W2058049327 creator A5011330390 @default.
- W2058049327 creator A5062934675 @default.
- W2058049327 date "1988-02-01" @default.
- W2058049327 modified "2023-09-26" @default.
- W2058049327 title "Identification of latent procathepsin H in microsomal lumen: Characterization of proteolytic processing and enzyme activation" @default.
- W2058049327 cites W1544813339 @default.
- W2058049327 cites W1577203384 @default.
- W2058049327 cites W1598109746 @default.
- W2058049327 cites W1761811954 @default.
- W2058049327 cites W1978867270 @default.
- W2058049327 cites W1992663882 @default.
- W2058049327 cites W2014922971 @default.
- W2058049327 cites W2058381769 @default.
- W2058049327 cites W2073394089 @default.
- W2058049327 cites W2077446180 @default.
- W2058049327 cites W2084862719 @default.
- W2058049327 cites W2101181033 @default.
- W2058049327 cites W2130898002 @default.
- W2058049327 cites W2137264529 @default.
- W2058049327 cites W4247894656 @default.
- W2058049327 doi "https://doi.org/10.1016/0003-9861(88)90500-0" @default.
- W2058049327 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3277536" @default.
- W2058049327 hasPublicationYear "1988" @default.
- W2058049327 type Work @default.
- W2058049327 sameAs 2058049327 @default.
- W2058049327 citedByCount "27" @default.
- W2058049327 countsByYear W20580493272012 @default.
- W2058049327 countsByYear W20580493272013 @default.
- W2058049327 crossrefType "journal-article" @default.
- W2058049327 hasAuthorship W2058049327A5011330390 @default.
- W2058049327 hasAuthorship W2058049327A5062934675 @default.
- W2058049327 hasConcept C145734084 @default.
- W2058049327 hasConcept C158617107 @default.
- W2058049327 hasConcept C173633252 @default.
- W2058049327 hasConcept C181199279 @default.
- W2058049327 hasConcept C185592680 @default.
- W2058049327 hasConcept C19869320 @default.
- W2058049327 hasConcept C202751555 @default.
- W2058049327 hasConcept C2776350231 @default.
- W2058049327 hasConcept C2776714187 @default.
- W2058049327 hasConcept C2779881004 @default.
- W2058049327 hasConcept C55493867 @default.
- W2058049327 hasConcept C87644729 @default.
- W2058049327 hasConceptScore W2058049327C145734084 @default.
- W2058049327 hasConceptScore W2058049327C158617107 @default.
- W2058049327 hasConceptScore W2058049327C173633252 @default.
- W2058049327 hasConceptScore W2058049327C181199279 @default.
- W2058049327 hasConceptScore W2058049327C185592680 @default.
- W2058049327 hasConceptScore W2058049327C19869320 @default.
- W2058049327 hasConceptScore W2058049327C202751555 @default.
- W2058049327 hasConceptScore W2058049327C2776350231 @default.
- W2058049327 hasConceptScore W2058049327C2776714187 @default.
- W2058049327 hasConceptScore W2058049327C2779881004 @default.
- W2058049327 hasConceptScore W2058049327C55493867 @default.
- W2058049327 hasConceptScore W2058049327C87644729 @default.
- W2058049327 hasLocation W20580493271 @default.
- W2058049327 hasLocation W20580493272 @default.
- W2058049327 hasOpenAccess W2058049327 @default.
- W2058049327 hasPrimaryLocation W20580493271 @default.
- W2058049327 hasRelatedWork W1602905293 @default.
- W2058049327 hasRelatedWork W1867141563 @default.
- W2058049327 hasRelatedWork W1957450506 @default.
- W2058049327 hasRelatedWork W2043814453 @default.
- W2058049327 hasRelatedWork W2053862558 @default.
- W2058049327 hasRelatedWork W2058049327 @default.
- W2058049327 hasRelatedWork W2061346437 @default.
- W2058049327 hasRelatedWork W2078259381 @default.
- W2058049327 hasRelatedWork W98911639 @default.
- W2058049327 hasRelatedWork W1987589598 @default.
- W2058049327 isParatext "false" @default.
- W2058049327 isRetracted "false" @default.
- W2058049327 magId "2058049327" @default.
- W2058049327 workType "article" @default.