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W2058054611 abstract "We developed a method to detect and identify proteins on a probe of the atomic force microscope (AFM) with a high sensitivity. Due to a low background noise of the total internal reflection fluorescence microscope employed as a detecting system, we were able to achieve a high enough sensitivity to detect zeptomole orders of protein molecules immobilized on the tip. Several different methods to immobilize protein molecules to AFM‐probes were tested, meant for a wide range of applications of this method. Furthermore, we demonstrated that different proteins were clearly distinguished by immunofluorescence microscopy on the probe using their specific antibodies." @default.
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W2058054611 date "2004-06-07" @default.
W2058054611 modified "2023-10-15" @default.
W2058054611 title "High sensitivity detection of protein molecules picked up on a probe of atomic force microscope based on the fluorescence detection by a total internal reflection fluorescence microscope" @default.
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W2058054611 doi "https://doi.org/10.1016/j.febslet.2004.05.049" @default.
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