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- W2058059969 abstract "Polymerase chain reaction (PCR) primers targeting genomic DNA of Xanthomonas campestris pv. citri, a causal agent of citrus bacterial canker, were designed for a rapid, sensitive and specific detection of the bacterium. The intergenic spacer region of approximately 500bp between the 16S and 23S rRNA genes from X. c. pv. citri, X. c. pv. glycines, X. c. pv. alfalfae, X. c. pv. physalidicola, X. c. pv. pisi, X. c. pv. pruni, X. c. pv. cucurbitae and X. c. pv. vesicatoria were sequenced. Subsequently, a pair of PCR primers, XCF (5'-AGGCCGGTATGCGAAAGTCCCATCA-3') and XCR (5'-CAAGTTGCCTCGGAGCT-ATC-3'), were designed on the basis of the sequence data of X. c. pv. citri, because these sequences differed from those of the other pathovars except X. c. pv. glycines. Although a fragment of 424bp was specifically amplified for all 36 isolates of X. c. pv. citri having different origins and for one isolate of X. c. pv. glycines, no PCR products were obtained from the other pathovars. To confirm specific detection of X. c. pv. citri, 81 bacteria isolated from citrus leaf surfaces were examined by PCR and pathogenicity tests. Consequently, PCR products were obtained from only nine pathogenic strains belonging to X. c. pv. citri, but no products were detected from the other non-pathogenic bacteria, indicating that X. c. pv. glycines is not an inhabitant of citrus orchard. The detectable limit of PCR amplification was 30cfu per ml, which was 10 times more sensitive than the leaf infiltration technique which had been considered the most sensitive. PCR using the specific primers designed in this study, was useful for rapid identification and detection of X. c. pv. citri from citrus samples within 6hr of sample collection." @default.
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- W2058059969 title "Detection of Xanthomonas campestris pv. citri by PCR Using Primers from the Spacer Region between the 16S and 23S rRNA Genes." @default.
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- W2058059969 doi "https://doi.org/10.3186/jjphytopath.64.249" @default.
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