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• W2058091725 abstract "An optimized capillary zone electrophoresis (CZE) method for the analysis of tetracycline (TC), chlortetracycline (CTC), oxytetracycline (OTC) and doxycycline (DXC) is described. Using fused-silica capillaries, the influence of the electrolyte composition, pH and concentration, as well as temperature and applied voltage were investigated. A factorial and central composite design was performed to optimize the method in a simple way. The optimal separation conditions were 50mmolL(-1) sodium carbonate+1mmolL(-1) EDTA, pH 10; voltage 13kV and temperature 23 degrees C. The method was validated for TC determination in pharmaceuticals through the following performance criteria: linearity and linear range, sensitivity, selectivity, intra-assay and inter-assay precision, detectability, accuracy and ruggedness. In comparison with the recommended HPLC method in the United States Pharmacopeia, this CZE-method exhibited the same performance as the official method, with the advantage that the same method could be used for the simultaneous determination of the different tetracyclines in pharmaceutical formulations." @default.
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• W2058091725 date "2006-09-15" @default.
• W2058091725 modified "2023-10-14" @default.
• W2058091725 title "Simultaneous determination of tetracyclines in pharmaceuticals by CZE using experimental design" @default.
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• W2058091725 doi "https://doi.org/10.1016/j.talanta.2006.02.048" @default.
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