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- W2058120758 abstract "The phytoalexin wyerone acid appeared to be metabolized by Botrytis fabae at a faster rate than by B. cinerea in vitro. Loss of wyerone acid caused by B. fabae, but not B. cinerea, was associated with the appearance of a novel u.v. absorbing substance (substance I). Substance I was purified by paper chromatography and found to behave as an ether soluble acid. The concentration of substance I in B. fabae infection droplets incubated in pod seed cavities increased rapidly between 1 and 3 days after inoculation. Substance I from B. fabae pod diffusate was purified by repeated t.l.c. and methylated to facilitate spectroscopic analyses. The metabolite was identified as reduced wyerone acid, and was much less antifungal to B. cinerea and B. fabae than wyerone acid. Substance I could not be detected in healthy tissues or large lesions caused by B. cinerea in broad bean leaves, but increased at B. fabae inoculation sites as tissues became blackened and colonized by B. fabae. No extracellular enzymes capable of degrading wyerone acid could be detected in diffusates from pods infected with B. fabae, suggesting that phytoalexin metabolism takes place within fungal hyphae. This work provides evidence that ability to metabolize phytoalexin may be an important characteristic of some phytopathogenic fungi." @default.
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- W2058120758 date "1973-07-01" @default.
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- W2058120758 title "The metabolism of wyerone acid (a phytoalexin from Vicia faba L.) by Botrytis fabae and B. cinerea" @default.
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- W2058120758 doi "https://doi.org/10.1016/0048-4059(73)90012-x" @default.
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