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- W2058158100 abstract "The effect of the exudates of Azotobacter chroococcum in nitrogen-free liquid medium and in sterile soil, on its ability to fix nitrogen was studied. Cell-free culture filtrates and water extract of sterile soil inoculated with Azotobacter were fractionated into ether soluble and insoluble aliquots, concentrated and chromatographically separated into ten consecutive zones. These were eluted and tested for their effect on nitrogen fixation by Azotobacter. UV-fluorescence, and chemical tests for indoles, reducing substances, amino acids, and hydroxyl groups were made. Detection of gibberellins and water soluble vitamins was attempted. A biological test for activity of indolic compounds was carried out.The results showed that Azotobacter produces biologically active indolic compounds in culture and soil, acting as a significant stimulation for the process of nitrogen fixation. Not all the zones of the chromatogram which were stimulatory for nitrogen fixation were the same for coleoptile growth and vice versa. Amino acids in some cases were associated with non-significant stimulation of nitrogen fixation. An indication was obtained suggesting the possible stimulation of nitrogen fixation by gibberellins produced in culture and soil by Azotobacter. Suggestion was given for studying the effect of individual gib berellic compounds on the nitrogen fixation process, with a view to their interaction with amino acids and indolic compounds." @default.
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- W2058158100 date "1972-01-01" @default.
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- W2058158100 title "Stimulatory effects ofAzotobacter chroococum exudates in culture and soil on its nitrogen fixation" @default.
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- W2058158100 doi "https://doi.org/10.1002/jobm.3630120204" @default.
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