FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058201675> ?p ?o ?g. }

• W2058201675 endingPage "27" @default.
• W2058201675 startingPage "27" @default.
• W2058201675 abstract "<p>Due to the urgent need of differentiation of infected from vaccinated animals in control and eradication of classical swine fever (CSF) and the shortcomings of current differential diagnostic tools, this study is aiming to establish a RT-nestPCR assay for differential detection of wild-type viruses and lapinized Chinese vaccine strain (C-strain) of classical swine fever virus (CSFV) of high sensitivity. Two pairs of CSFV-specific primers were designed in the conservative regions of NS5B (a non-structural protein encoded by the CSFV genome, which performs the RNA dependent RNA polymerase activity) and 3? un-translated regions (3?-UTR) to encompass the T-rich insertion uniquely existing in the 3?-UTR of C-strain genome. Thus the amplification fragment of C-strain is longer than that of the wild-type viruses for it contains the T-rich insertion region. Two pairs of primers were used in combination and the wild-type viruses and C-strain of CSFV could be detected and accurately distinguished with a high sensitivity through super fine resolution (SFR) argarose gel electrophoresis that displays the different lengths of the amplicons. The detection limit of the C-strain and Shimen strain were respectively 4.5×10^-2pg and 3.2×10^-2pg of viral RNA. The results of the specificity test showed that this method can detect different strains of CSFV without amplifying other non-CSFV pathogens. The results of the detection of 400 clinical samples indicated that 16 samples were CSFV positive in total; in which 4 samples were C-Strain positive and 12 were wild-type CSFV positive. The total CSFV positive rate was 4%. The detection results of the 14 batches of C-Strain vaccines showed that all samples displayed bands of C-Strain amplicons in the SFR argarose gel electrophoresis and all vaccines were free of wild-type virus contamination. In conclusion, the RT-nestPCR assay established in the present study could supply a sensitive and specific test method for distinguishing wild-type CSFV infected animals from those vaccinated with C-strain vaccines in the field.</p>" @default.
• W2058201675 created "2016-06-24" @default.
• W2058201675 creator A5007464615 @default.
• W2058201675 creator A5030068214 @default.
• W2058201675 creator A5063635496 @default.
• W2058201675 creator A5064069208 @default.
• W2058201675 creator A5081165104 @default.
• W2058201675 creator A5090824448 @default.
• W2058201675 date "2013-04-06" @default.
• W2058201675 modified "2023-10-02" @default.
• W2058201675 title "Development and Application of a RT-NestPCR Assay for Differential Detection of C-Strain and Wild-Type Viruses of Classical Swine Fever Virus" @default.
• W2058201675 cites W1608407313 @default.
• W2058201675 cites W1965506032 @default.
• W2058201675 cites W1967778241 @default.
• W2058201675 cites W1969202289 @default.
• W2058201675 cites W1973010334 @default.
• W2058201675 cites W1975009152 @default.
• W2058201675 cites W1984119182 @default.
• W2058201675 cites W1986765760 @default.
• W2058201675 cites W1990929600 @default.
• W2058201675 cites W1996824979 @default.
• W2058201675 cites W1998178430 @default.
• W2058201675 cites W1999573683 @default.
• W2058201675 cites W2024080387 @default.
• W2058201675 cites W2026030211 @default.
• W2058201675 cites W2033469947 @default.
• W2058201675 cites W2035805381 @default.
• W2058201675 cites W2036444482 @default.
• W2058201675 cites W2038470481 @default.
• W2058201675 cites W2039236914 @default.
• W2058201675 cites W2045093985 @default.
• W2058201675 cites W2055043387 @default.
• W2058201675 cites W2057833008 @default.
• W2058201675 cites W2059691183 @default.
• W2058201675 cites W2061951215 @default.
• W2058201675 cites W2071416171 @default.
• W2058201675 cites W2080106938 @default.
• W2058201675 cites W2081014948 @default.
• W2058201675 cites W2106090606 @default.
• W2058201675 cites W2120389910 @default.
• W2058201675 cites W2132571290 @default.
• W2058201675 cites W2134822561 @default.
• W2058201675 cites W2137917626 @default.
• W2058201675 cites W2137977382 @default.
• W2058201675 cites W2146348920 @default.
• W2058201675 cites W2147651897 @default.
• W2058201675 cites W2166471331 @default.
• W2058201675 cites W2170488050 @default.
• W2058201675 cites W2430534168 @default.
• W2058201675 cites W2470593998 @default.
• W2058201675 cites W274045972 @default.
• W2058201675 cites W27431358 @default.
• W2058201675 doi "https://doi.org/10.5539/sar.v2n3p27" @default.
• W2058201675 hasPublicationYear "2013" @default.
• W2058201675 type Work @default.
• W2058201675 sameAs 2058201675 @default.
• W2058201675 citedByCount "0" @default.
• W2058201675 crossrefType "journal-article" @default.
• W2058201675 hasAuthorship W2058201675A5007464615 @default.
• W2058201675 hasAuthorship W2058201675A5030068214 @default.
• W2058201675 hasAuthorship W2058201675A5063635496 @default.
• W2058201675 hasAuthorship W2058201675A5064069208 @default.
• W2058201675 hasAuthorship W2058201675A5081165104 @default.
• W2058201675 hasAuthorship W2058201675A5090824448 @default.
• W2058201675 hasBestOaLocation W20582016751 @default.
• W2058201675 hasConcept C104317684 @default.
• W2058201675 hasConcept C105702510 @default.
• W2058201675 hasConcept C141231307 @default.
• W2058201675 hasConcept C153911025 @default.
• W2058201675 hasConcept C159047783 @default.
• W2058201675 hasConcept C2522874641 @default.
• W2058201675 hasConcept C2777510210 @default.
• W2058201675 hasConcept C2778022156 @default.
• W2058201675 hasConcept C2778400998 @default.
• W2058201675 hasConcept C2779005923 @default.
• W2058201675 hasConcept C2780727368 @default.
• W2058201675 hasConcept C49105822 @default.
• W2058201675 hasConcept C54355233 @default.
• W2058201675 hasConcept C8185291 @default.
• W2058201675 hasConcept C86803240 @default.
• W2058201675 hasConceptScore W2058201675C104317684 @default.
• W2058201675 hasConceptScore W2058201675C105702510 @default.
• W2058201675 hasConceptScore W2058201675C141231307 @default.
• W2058201675 hasConceptScore W2058201675C153911025 @default.
• W2058201675 hasConceptScore W2058201675C159047783 @default.
• W2058201675 hasConceptScore W2058201675C2522874641 @default.
• W2058201675 hasConceptScore W2058201675C2777510210 @default.
• W2058201675 hasConceptScore W2058201675C2778022156 @default.
• W2058201675 hasConceptScore W2058201675C2778400998 @default.
• W2058201675 hasConceptScore W2058201675C2779005923 @default.
• W2058201675 hasConceptScore W2058201675C2780727368 @default.
• W2058201675 hasConceptScore W2058201675C49105822 @default.
• W2058201675 hasConceptScore W2058201675C54355233 @default.
• W2058201675 hasConceptScore W2058201675C8185291 @default.
• W2058201675 hasConceptScore W2058201675C86803240 @default.
• W2058201675 hasIssue "3" @default.
• W2058201675 hasLocation W20582016751 @default.
• W2058201675 hasLocation W20582016752 @default.

• next