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- W2058215944 abstract "Orotate phosphoribosyltransferase (OPRT) is a key enzyme in the anabolism of 5-fluorouracil (5-FU), and its expression in tumors is thought to increase the efficacy of 5-FU against the tumor. To detect the OPRT protein by immunoblot and/or immunohistochemical methods, we prepared highly specific antibody to the peptides contained in the human OPRT amino acid sequence. The anti-OPRT polyclonal antibodies, obtained by immunizing rabbits with the OPRT peptides, had a high specificity for the OPRT protein in human tumor xenografts when it was analyzed by immunoblotting, and furthermore, there was a positive correlation between OPRT activity and protein content in 12 human tumors (R2 = 0.632). These results suggest that immunohistochemical detection of tumoral OPRT protein expression with our anti-OPRT antibodies may provide a useful method for predicting the clinical response to 5-FU-based chemotherapy." @default.
- W2058215944 created "2016-06-24" @default.
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- W2058215944 date "2005-08-01" @default.
- W2058215944 modified "2023-09-25" @default.
- W2058215944 title "Preparation of anti-orotate phosphoribosyltransferase antibody and its application to immunochemical detection in human tumor cells" @default.
- W2058215944 doi "https://doi.org/10.3892/ijmm.16.2.245" @default.
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