FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058219098> ?p ?o ?g. }

• W2058219098 endingPage "93" @default.
• W2058219098 startingPage "83" @default.
• W2058219098 abstract "In the yeast Saccharomyces cerevisiae inactivation of trehalose-6-phosphate (Tre6P) synthase (Tps1) encoded by the TPS1 gene causes a specific growth defect in the presence of glucose in the medium. The growth inhibition is associated with deregulation of the initial part of glycolysis. Sugar phosphates, especially fructose-1,6-bisphosphate (Fru1,6bisP), hyperaccumulate while the levels of ATP, Pi and downstream metabolites are rapidly depleted. This was suggested to be due to the absence of Tre6P inhibition on hexokinase. Here we show that overexpression of Tre6P (as well as glucose-6-phosphate (Glu6P))-insensitive hexokinase from Schizosaccharomyces pombe in a wild-type strain does not affect growth on glucose but still transiently enhances initial sugar phosphate accumulation. We have in addition replaced the three endogenous glucose kinases of S. cerevisiae by the Tre6P-insensitive hexokinase from S. pombe. High hexokinase activity was measured in cell extracts and growth on glucose was somewhat reduced compared to an S. cerevisiae wild-type strain but expression of the Tre6P-insensitive S. pombe hexokinase never caused the typical tps1Delta phenotype. Moreover, deletion of TPS1 in this strain expressing only the Tre6P-insensitive S. pombe hexokinase still resulted in a severe drop in growth capacity on glucose as well as sensitivity to millimolar glucose levels in the presence of excess galactose. In this case, poor growth on glucose was associated with reduced rather than enhanced glucose influx into glycolysis. Initial glucose transport was not affected. Apparently, deletion of TPS1 causes reduced activity of the S. pombe hexokinase in vivo. Our results show that Tre6P inhibition of hexokinase is not the major mechanism by which Tps1 controls the influx of glucose into glycolysis or the capacity to grow on glucose. In addition, they show that a Tre6P-insensitive hexokinase can still be controlled by Tps1 in vivo." @default.
• W2058219098 created "2016-06-24" @default.
• W2058219098 creator A5019737118 @default.
• W2058219098 creator A5035778660 @default.
• W2058219098 creator A5090068893 @default.
• W2058219098 date "2003-09-01" @default.
• W2058219098 modified "2023-10-17" @default.
• W2058219098 title "Uncoupling of the glucose growth defect and the deregulation of glycolysis in Saccharomyces cerevisiae tps1 mutants expressing trehalose-6-phosphate-insensitive hexokinase from Schizosaccharomyces pombe" @default.
• W2058219098 cites W1456663196 @default.
• W2058219098 cites W1504082382 @default.
• W2058219098 cites W1526477746 @default.
• W2058219098 cites W1544121454 @default.
• W2058219098 cites W1559442263 @default.
• W2058219098 cites W1605292371 @default.
• W2058219098 cites W1677466026 @default.
• W2058219098 cites W1931112564 @default.
• W2058219098 cites W1937798450 @default.
• W2058219098 cites W1965254867 @default.
• W2058219098 cites W1966170707 @default.
• W2058219098 cites W1968399116 @default.
• W2058219098 cites W1968917541 @default.
• W2058219098 cites W1968918655 @default.
• W2058219098 cites W1976254673 @default.
• W2058219098 cites W1989229525 @default.
• W2058219098 cites W1990520393 @default.
• W2058219098 cites W1991218861 @default.
• W2058219098 cites W1991703531 @default.
• W2058219098 cites W1995956297 @default.
• W2058219098 cites W1996119276 @default.
• W2058219098 cites W2004829905 @default.
• W2058219098 cites W2016370480 @default.
• W2058219098 cites W2017205827 @default.
• W2058219098 cites W2020203784 @default.
• W2058219098 cites W2024785422 @default.
• W2058219098 cites W2024881381 @default.
• W2058219098 cites W2028897922 @default.
• W2058219098 cites W2032264774 @default.
• W2058219098 cites W2033507393 @default.
• W2058219098 cites W2036991355 @default.
• W2058219098 cites W2037296779 @default.
• W2058219098 cites W2055548976 @default.
• W2058219098 cites W2060218165 @default.
• W2058219098 cites W2066776652 @default.
• W2058219098 cites W2069997924 @default.
• W2058219098 cites W2070760055 @default.
• W2058219098 cites W2072428163 @default.
• W2058219098 cites W2077082936 @default.
• W2058219098 cites W2080365239 @default.
• W2058219098 cites W2086968057 @default.
• W2058219098 cites W2089782157 @default.
• W2058219098 cites W210226531 @default.
• W2058219098 cites W2119055631 @default.
• W2058219098 cites W2125754059 @default.
• W2058219098 cites W2161905646 @default.
• W2058219098 cites W2170371323 @default.
• W2058219098 cites W2888026230 @default.
• W2058219098 cites W903917906 @default.
• W2058219098 doi "https://doi.org/10.1016/s0005-2728(03)00086-0" @default.
• W2058219098 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14507429" @default.
• W2058219098 hasPublicationYear "2003" @default.
• W2058219098 type Work @default.
• W2058219098 sameAs 2058219098 @default.
• W2058219098 citedByCount "38" @default.
• W2058219098 countsByYear W20582190982013 @default.
• W2058219098 countsByYear W20582190982014 @default.
• W2058219098 countsByYear W20582190982015 @default.
• W2058219098 countsByYear W20582190982016 @default.
• W2058219098 countsByYear W20582190982017 @default.
• W2058219098 countsByYear W20582190982020 @default.
• W2058219098 countsByYear W20582190982022 @default.
• W2058219098 countsByYear W20582190982023 @default.
• W2058219098 crossrefType "journal-article" @default.
• W2058219098 hasAuthorship W2058219098A5019737118 @default.
• W2058219098 hasAuthorship W2058219098A5035778660 @default.
• W2058219098 hasAuthorship W2058219098A5090068893 @default.
• W2058219098 hasBestOaLocation W20582190981 @default.
• W2058219098 hasConcept C134018914 @default.
• W2058219098 hasConcept C161573976 @default.
• W2058219098 hasConcept C181199279 @default.
• W2058219098 hasConcept C20251656 @default.
• W2058219098 hasConcept C2776519988 @default.
• W2058219098 hasConcept C2777576037 @default.
• W2058219098 hasConcept C2777790778 @default.
• W2058219098 hasConcept C2778452849 @default.
• W2058219098 hasConcept C2779222958 @default.
• W2058219098 hasConcept C2779306644 @default.
• W2058219098 hasConcept C2779429346 @default.
• W2058219098 hasConcept C2779826832 @default.
• W2058219098 hasConcept C55493867 @default.
• W2058219098 hasConcept C62231903 @default.
• W2058219098 hasConcept C86803240 @default.
• W2058219098 hasConcept C89200155 @default.
• W2058219098 hasConcept C95444343 @default.
• W2058219098 hasConceptScore W2058219098C134018914 @default.
• W2058219098 hasConceptScore W2058219098C161573976 @default.
• W2058219098 hasConceptScore W2058219098C181199279 @default.
• W2058219098 hasConceptScore W2058219098C20251656 @default.
• W2058219098 hasConceptScore W2058219098C2776519988 @default.

• next