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• W2058231486 abstract "More than 3000 myocardial protein species of Wistar Kyoto rat, an important animal model, were separated by high-resolution two-dimensional gel electrophoresis (2-DE) and characterized in terms of isoelectric point (pI) and molecular mass (Mr). Currently, the 2-DE database contains 64 identified proteins; forty-three were identified by peptide mass fingerprinting (PMF) using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), nine by exclusive comparison with other 2-DE heart protein databases, and in only 12 cases of 60 attempts N-terminal sequencing was successful. We used the Make2ddb software package downloaded from the ExPASy server for the construction of a rat myocardial 2-DE database. The Make2ddb package simplifies the creation of a new 2-DE database if the Melanie II software and a Sun workstation under Solaris are available. Our 2-DE database of rat heart proteins can be accessed at URL http://gelmatching.inf.fu-berlin.de/˜pleiss/2d." @default.
• W2058231486 created "2016-06-24" @default.
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• W2058231486 date "1999-01-01" @default.
• W2058231486 modified "2023-10-05" @default.
• W2058231486 title "A two-dimensional electrophoresis database of rat heart proteins" @default.
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• W2058231486 doi "https://doi.org/10.1002/(sici)1522-2683(19990101)20:4/5<891::aid-elps891>3.0.co;2-2" @default.
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