Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058243578> ?p ?o ?g. }
- W2058243578 endingPage "517" @default.
- W2058243578 startingPage "510" @default.
- W2058243578 abstract "Methotrexate (MTX) is a dihydrofolate reductase (DHFR) inhibitor widely used as an anticancer drug in different kinds of human cancers. Here we investigated the anti-tumor mechanism of MTX against non-small cell lung cancer (NSCLC) A549 cells. MTX not only inhibited in vitro cell growth via induction of apoptosis, but also inhibited tumor formation in animal xenograft model. RNase protection assay (RPA) and RT-PCR demonstrated its induction of p53 target genes including DR5, p21, Puma and Noxa. Moreover, MTX promoted p53 phosphorylation at Ser15 and acetylaion at Lys373/382, which increase its stability and expression. The apoptosis and inhibition of cell viability induced by MTX were dependent on p53 and, partially, on p21. In addition, MTX also increased E-cadherin expression through inhibition of histone deacetylase (HDAC) activity and downregulation of polycomb group protein enhancer of zeste homologue 2 (EZH2). Therefore, the anticancer mechanism of MTX acts through initiation of p53-dependent apoptosis and restoration of E-cadherin expression by downregulation of HDAC/EZH2." @default.
- W2058243578 created "2016-06-24" @default.
- W2058243578 creator A5007763690 @default.
- W2058243578 creator A5024717098 @default.
- W2058243578 creator A5034407820 @default.
- W2058243578 creator A5038563409 @default.
- W2058243578 creator A5067617421 @default.
- W2058243578 date "2011-02-01" @default.
- W2058243578 modified "2023-10-18" @default.
- W2058243578 title "Methotrexate induces apoptosis through p53/p21-dependent pathway and increases E-cadherin expression through downregulation of HDAC/EZH2" @default.
- W2058243578 cites W1510180204 @default.
- W2058243578 cites W1548972556 @default.
- W2058243578 cites W1937607074 @default.
- W2058243578 cites W1967018540 @default.
- W2058243578 cites W1972149587 @default.
- W2058243578 cites W1976873804 @default.
- W2058243578 cites W1977090021 @default.
- W2058243578 cites W1978172257 @default.
- W2058243578 cites W1983684591 @default.
- W2058243578 cites W1984971509 @default.
- W2058243578 cites W1992109541 @default.
- W2058243578 cites W1996487270 @default.
- W2058243578 cites W1999517490 @default.
- W2058243578 cites W2000816728 @default.
- W2058243578 cites W2008887283 @default.
- W2058243578 cites W2022431825 @default.
- W2058243578 cites W2024873523 @default.
- W2058243578 cites W2030149741 @default.
- W2058243578 cites W2037583144 @default.
- W2058243578 cites W2038116705 @default.
- W2058243578 cites W2045837572 @default.
- W2058243578 cites W2049763628 @default.
- W2058243578 cites W2062778788 @default.
- W2058243578 cites W2063669247 @default.
- W2058243578 cites W2070136352 @default.
- W2058243578 cites W2071555702 @default.
- W2058243578 cites W2080125477 @default.
- W2058243578 cites W2082541302 @default.
- W2058243578 cites W2082979850 @default.
- W2058243578 cites W2083627679 @default.
- W2058243578 cites W2089082314 @default.
- W2058243578 cites W2100365251 @default.
- W2058243578 cites W2104209180 @default.
- W2058243578 cites W2112287022 @default.
- W2058243578 cites W2113634596 @default.
- W2058243578 cites W2121737942 @default.
- W2058243578 cites W2125730874 @default.
- W2058243578 cites W2126701902 @default.
- W2058243578 cites W2128844255 @default.
- W2058243578 cites W2146916084 @default.
- W2058243578 cites W2147723796 @default.
- W2058243578 cites W2150416393 @default.
- W2058243578 cites W2154986076 @default.
- W2058243578 cites W2156596286 @default.
- W2058243578 cites W2161930186 @default.
- W2058243578 cites W2162647319 @default.
- W2058243578 cites W2167620731 @default.
- W2058243578 cites W2330189712 @default.
- W2058243578 doi "https://doi.org/10.1016/j.bcp.2010.11.014" @default.
- W2058243578 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21114963" @default.
- W2058243578 hasPublicationYear "2011" @default.
- W2058243578 type Work @default.
- W2058243578 sameAs 2058243578 @default.
- W2058243578 citedByCount "46" @default.
- W2058243578 countsByYear W20582435782012 @default.
- W2058243578 countsByYear W20582435782013 @default.
- W2058243578 countsByYear W20582435782014 @default.
- W2058243578 countsByYear W20582435782015 @default.
- W2058243578 countsByYear W20582435782016 @default.
- W2058243578 countsByYear W20582435782017 @default.
- W2058243578 countsByYear W20582435782018 @default.
- W2058243578 countsByYear W20582435782019 @default.
- W2058243578 countsByYear W20582435782020 @default.
- W2058243578 countsByYear W20582435782021 @default.
- W2058243578 countsByYear W20582435782022 @default.
- W2058243578 countsByYear W20582435782023 @default.
- W2058243578 crossrefType "journal-article" @default.
- W2058243578 hasAuthorship W2058243578A5007763690 @default.
- W2058243578 hasAuthorship W2058243578A5024717098 @default.
- W2058243578 hasAuthorship W2058243578A5034407820 @default.
- W2058243578 hasAuthorship W2058243578A5038563409 @default.
- W2058243578 hasAuthorship W2058243578A5067617421 @default.
- W2058243578 hasConcept C104317684 @default.
- W2058243578 hasConcept C127561419 @default.
- W2058243578 hasConcept C153911025 @default.
- W2058243578 hasConcept C185592680 @default.
- W2058243578 hasConcept C190283241 @default.
- W2058243578 hasConcept C22615655 @default.
- W2058243578 hasConcept C2777417711 @default.
- W2058243578 hasConcept C2778305200 @default.
- W2058243578 hasConcept C29537977 @default.
- W2058243578 hasConcept C50171091 @default.
- W2058243578 hasConcept C502942594 @default.
- W2058243578 hasConcept C53227056 @default.
- W2058243578 hasConcept C54009773 @default.
- W2058243578 hasConcept C55493867 @default.
- W2058243578 hasConcept C62112901 @default.
- W2058243578 hasConcept C64927066 @default.