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- W2058267257 abstract "Some basic components of an indirect competitive enzyme-linked immunosorbent assay (ELISA) were manipulated to reduce sulfathiazole (ST) detection limits. 3.3′.5.5′-Tetramethvlbenzidine (TMB) was more kffeitive than several aliemative chromogens tested for the hetection of horseradish peroxidase activity. Color development was maximized at pH 4.0. Using TMB and a 2M sulfuric acid stopping reagent, an assay was develoued using highly diluted (1:500,000) rabbit serum and low levels (0.0313 μg/mY.), of coating conjugate, with a C value (inflection point of sigmoid standard curve) of 4.4 ppb ST. Urease was unsuitable as a marker for indirect competitive ELISAs." @default.
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- W2058267257 date "1995-07-01" @default.
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- W2058267257 title "Variation in Enzyme-linked lmmunosorbent Assay (ELISA) Components to Lower Sulfathiazole Detection Limits" @default.
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- W2058267257 doi "https://doi.org/10.1111/j.1365-2621.1995.tb06250.x" @default.
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