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- W2058303611 abstract "(A) Overexpressed NIK and ASK1proteins bind directly. HA-tagged ASK1 and FLAG-NIK were tran-siently co-transfected into BOSC 23 cells, and the lysates were immuno-precipitated with appropriate antibodies. Samples were subjected to Western blotting analysis using appropriate antibodies. The expressionof transfected plasmids were indicated in the same lysates. IP, immuno-precipitation; IB, immunoblot. (B) NIK binds to the C-terminal domai nof ASK1. BOSC 23 cells were transiently co-transfected with FLAG-tagged NIK and HA-tagged ASK1 wild-type or mutant expression plasmids. Transfected cells were extracted and immunoprecipitated with an anti-HA antibody. The interaction was detected by Western blotting with an anti-FLAG antibody." @default.
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- W2058303611 date "2010-07-20" @default.
- W2058303611 modified "2023-10-18" @default.
- W2058303611 title "Regulation of NF-κB signaling by ASK1 through interaction with NIK" @default.
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- W2058303611 doi "https://doi.org/10.5012/bkcs.2010.31.7.2110" @default.
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