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- W2058320971 abstract "Mammals can be molecular sexed by polymerase chain reaction (PCR) amplification of Y chromosome fragments or coamplification of homologous fragments from both sex chromosomes, which are discriminated by size polymorphism or Y-specific restriction digestion. Although coamplification of X and Y fragments is more reliable, size polymorphism in homologous fragments is uncommon and Y-specific restriction site identification requires screening with a battery of enzymes or cloning. Here we describe a simple approach, using ‘double peaks’ in the chromatogram upon direct sequencing of PCR products from males, to identify Y-specific restriction sites, and demonstrate its utility by application to a range of taxa." @default.
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- W2058320971 date "2001-12-01" @default.
- W2058320971 modified "2023-10-11" @default.
- W2058320971 title "Molecular sexing eutherian mammals" @default.
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- W2058320971 doi "https://doi.org/10.1046/j.1471-8278.2001.00112.x" @default.
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