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- W2058339818 abstract "Our previous studies isolated and characterized eight splicing variants containing exon 11 as the first coding exon. The location of exon 11 about 10 kb upstream from exon 1 dual promoters implied another promoter to drive expression of the exon 11 variants. I now identify the second promoter in the 5′-flanking region of exon 11. One major transcriptional start point was determined. Sequence analysis indicated that the 5′-flanking region of the exon 11 contained a putative TATA box, several CAAT boxes and a number of cis-acting elements. Functional analysis suggested that exon 11 promoter activity was most evident in neuronal-like cells. A basal core region containing the TATA box, a negative region and a positive region were identified. Electrophoretic mobility shift assays with the nuclear extracts from NIE-115 cells revealed several protein complexes that likely contained TATA box-associated factors, NF-1-like and cMyc-Max-like proteins, respectively. It also showed that a TATA-binding protein specifically bound to the TATA box fragment. Mutation analysis suggested that the TATA box in the basal core region played a fundamental role in the exon 11 promoter, whereas the NF-1 site acted as a positive element." @default.
- W2058339818 created "2016-06-24" @default.
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- W2058339818 date "2002-07-01" @default.
- W2058339818 modified "2023-10-12" @default.
- W2058339818 title "Identification and characterization of a novel promoter of the mouse mu opioid receptor gene (Oprm) that generates eight splice variants" @default.
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- W2058339818 doi "https://doi.org/10.1016/s0378-1119(02)00825-9" @default.
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