Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058385108> ?p ?o ?g. }
- W2058385108 endingPage "998" @default.
- W2058385108 startingPage "985" @default.
- W2058385108 abstract "We have analyzed P-element-transformed lines carrying hsp26/lacZ transgenes with various deletions and substitutions within the Drosophila melanogaster hsp26 promoter region in order to identify the sequences required for the formation of the DNase I hypersensitive sites (DH sites). DH sites are generally found associated with promoters and enhancer elements of active and inducible eukaryotic genes, and are thought to be nucleosome-free regions of DNA that interact with regulatory proteins and the transcriptional machinery. There are two major DH sites located within the promoter region of the hsp26 gene, centered at −50 and at −350 (relative to the hsp26 transcription start site). The sequences from −135 to −85, which contain (CT)n · (GA)n repeats, contribute significantly to the formation of the DH sites in the hsp26 promoter region. Deletion or substitution of this (CT)n region drastically reduces the accessibility of the DNA at these sites to DNase I. This reduction in accessibility was quantified by measuring the susceptibility of the DNA within nuclei to cleavage at a restriction site within the DH site. In addition to the (CT)n region and the promoter at −85 to +11 (region P), one of two other regions must be present for effective creation of the DH sites: sequences between −351 and −135 (region A), or sequences between +11 and +632 (region D). Disruption of the wild-type chromatin structure, as assayed by the loss of accessibility to the DH sites, is correlated with a decrease in inducible transcriptional activity, even when the TATA box and heat shock regulatory elements are present in their normal positions." @default.
- W2058385108 created "2016-06-24" @default.
- W2058385108 creator A5009841533 @default.
- W2058385108 creator A5019603661 @default.
- W2058385108 creator A5054413005 @default.
- W2058385108 creator A5058864928 @default.
- W2058385108 creator A5060183257 @default.
- W2058385108 creator A5086056549 @default.
- W2058385108 date "1992-06-01" @default.
- W2058385108 modified "2023-10-16" @default.
- W2058385108 title "Promoter sequence containing (CT)n · (GA)n repeats is critical for the formation of the DNase I hypersensitive sites in the Drosophila hsp26 gene" @default.
- W2058385108 cites W1493507586 @default.
- W2058385108 cites W1554360427 @default.
- W2058385108 cites W1599809900 @default.
- W2058385108 cites W1637308698 @default.
- W2058385108 cites W1849437098 @default.
- W2058385108 cites W1960503626 @default.
- W2058385108 cites W1967023402 @default.
- W2058385108 cites W1969638997 @default.
- W2058385108 cites W1980014349 @default.
- W2058385108 cites W1994528729 @default.
- W2058385108 cites W1999939543 @default.
- W2058385108 cites W2001369720 @default.
- W2058385108 cites W2007878997 @default.
- W2058385108 cites W2009968835 @default.
- W2058385108 cites W2013812530 @default.
- W2058385108 cites W2014669590 @default.
- W2058385108 cites W2015464611 @default.
- W2058385108 cites W2023491365 @default.
- W2058385108 cites W2025060557 @default.
- W2058385108 cites W2026735932 @default.
- W2058385108 cites W2041309113 @default.
- W2058385108 cites W2042491847 @default.
- W2058385108 cites W2042543706 @default.
- W2058385108 cites W2044539033 @default.
- W2058385108 cites W2048374199 @default.
- W2058385108 cites W2081088219 @default.
- W2058385108 cites W2081495213 @default.
- W2058385108 cites W2085380563 @default.
- W2058385108 cites W2089787306 @default.
- W2058385108 cites W2091228527 @default.
- W2058385108 cites W2102131815 @default.
- W2058385108 cites W2118101941 @default.
- W2058385108 cites W2135621961 @default.
- W2058385108 cites W2137508075 @default.
- W2058385108 cites W2167110107 @default.
- W2058385108 cites W2172305010 @default.
- W2058385108 cites W2177510297 @default.
- W2058385108 cites W224702310 @default.
- W2058385108 cites W272039099 @default.
- W2058385108 cites W319346235 @default.
- W2058385108 cites W322623296 @default.
- W2058385108 cites W4233003623 @default.
- W2058385108 cites W4248066889 @default.
- W2058385108 cites W73481865 @default.
- W2058385108 doi "https://doi.org/10.1016/0022-2836(92)90099-6" @default.
- W2058385108 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1377279" @default.
- W2058385108 hasPublicationYear "1992" @default.
- W2058385108 type Work @default.
- W2058385108 sameAs 2058385108 @default.
- W2058385108 citedByCount "73" @default.
- W2058385108 countsByYear W20583851082013 @default.
- W2058385108 countsByYear W20583851082021 @default.
- W2058385108 crossrefType "journal-article" @default.
- W2058385108 hasAuthorship W2058385108A5009841533 @default.
- W2058385108 hasAuthorship W2058385108A5019603661 @default.
- W2058385108 hasAuthorship W2058385108A5054413005 @default.
- W2058385108 hasAuthorship W2058385108A5058864928 @default.
- W2058385108 hasAuthorship W2058385108A5060183257 @default.
- W2058385108 hasAuthorship W2058385108A5086056549 @default.
- W2058385108 hasBestOaLocation W20583851082 @default.
- W2058385108 hasConcept C101762097 @default.
- W2058385108 hasConcept C104317684 @default.
- W2058385108 hasConcept C111936080 @default.
- W2058385108 hasConcept C138885662 @default.
- W2058385108 hasConcept C150194340 @default.
- W2058385108 hasConcept C153911025 @default.
- W2058385108 hasConcept C165864922 @default.
- W2058385108 hasConcept C179926584 @default.
- W2058385108 hasConcept C182614070 @default.
- W2058385108 hasConcept C21592294 @default.
- W2058385108 hasConcept C2779377703 @default.
- W2058385108 hasConcept C2780104201 @default.
- W2058385108 hasConcept C3017666073 @default.
- W2058385108 hasConcept C41895202 @default.
- W2058385108 hasConcept C54355233 @default.
- W2058385108 hasConcept C552990157 @default.
- W2058385108 hasConcept C58237431 @default.
- W2058385108 hasConcept C65888428 @default.
- W2058385108 hasConcept C83640560 @default.
- W2058385108 hasConcept C84772758 @default.
- W2058385108 hasConcept C86339819 @default.
- W2058385108 hasConcept C86803240 @default.
- W2058385108 hasConceptScore W2058385108C101762097 @default.
- W2058385108 hasConceptScore W2058385108C104317684 @default.
- W2058385108 hasConceptScore W2058385108C111936080 @default.
- W2058385108 hasConceptScore W2058385108C138885662 @default.
- W2058385108 hasConceptScore W2058385108C150194340 @default.